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We narrowed to 470 results for: mag

Showing: 121 - 140 of 470 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon microscopy to detect action...was ASAP5 able to detect mEPSPs, it also enabled imaging of their propagation along dendrites for the first...also used AAV9 to deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through...to 600 µm. Figure 3: ASAP5 for voltage imaging. A) ASAP5 selected for its improved kinetics and...non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 ...

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... used for recombinant antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it ...biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are incubated with...suspension. Antibodies bind ligands on the beads and a magnet is used to separate the beads from the media (Brechmann... and the concentration may be calculated using imaging software. Any bands on the gel beside the heavy... Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension. ...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ...expressed. Cells are then cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI...sequence derived either from p63(1-29) and Lck. Image from Chertkova et al., 2020. In their BioRxiv... adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow of nanobody expression and...genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker et al., 2020. The lab tested ...wavelengths above 1,000 nm and enable two-color imaging with red fluorescence protein-based calcium sensors...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...photobleaching method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching...destroy the donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed more ...acceptor. It should be noted that FLIM is a slower imaging method, limiting its use in many FRET experiments...interpreting data. FRET is 20 years old but as you can imagine not old-fashioned at all. With the development ...Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol...

  5. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...the fused XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals only permits...fluorophores and that can be combined with new imaging techniques.  References Cai, Dawen, et al. "Improved...17972876. Pan, Y. Albert, et al. "Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1...Addgene blog Read an introduction to live cell imaging Learn more about the origin of the Brainbow system...collection Learn about fluorescent tools for in vivo imaging ...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...trend in biology towards single-cell resolution imaging. Sanes and Lichtman endeavored to create a map ... these Brainbow constructs into mice. When they imaged the mouse brains, they found many more than 3-4...suffered from a number of limitations. First, the imaging of Brainbow mouse tissue was challenging due to...described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine Loulier, et ...given section of brain by at least an order of magnitude. It is clear that further refinements of the Brainbow...

  7. Technical Design of a Western Blot

    Type
    Blog Post
    ...months at 4 °C.  Pro tip! If you get a speckled image, your protein-based blocker is either degraded or...to be compatible with stripping and probing.   Imaging At the final step of your western, you’ll have ...cost, but require no consumables, produce digital images, reach their limit of detection quickly (~1 minute...dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent...Pro tip! You only need a resolution of ~150 µm to image a blot. This is because proteins diffuse semiradially...-based buffers Increased cost X-ray film imaging Increased time; increased consumables cost ...

  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...recovered from seven rescued F1 animals (gel image under worm). Image courtesy of Jordan Ward, UCSF. In ...2xFLAG knock-in by direct screening. The center images depict the power of lethal mutation co-selection...CRISPRa), visualize specific genomic loci (CRISPR-imaging), or to find the proteins associated with a given...could further transform the range of experiments imaginable in C. elegans. Equally, some of the “elegans”...

  9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ... et al., 2008; Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs...1984; Van Duyne, 2001; Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors contain a strong... Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping." Journal of Neuroscience...Central PMCID: PMC4971574. Hooper, Andrew, and Jamie Maguire. "Characterization of a novel subtype of hippocampal...11340053. Wick, Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development...

  10. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution). I have worked...microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies naturally...frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic reporter genes’ on our...et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts." Nature 553.7686...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et al...clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com. Abbreviations: AAV, ...This capsid was developed and deposited by the Maguire lab. In mice, it is sex-independent and does not...similar to humans than New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. ...Tsoulfas, P., & Chaudhari, N. (2022). Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP....D., Dujardin, S., Ramirez, S. H., Hudry, E., & Maguire, C. A. (2019). Selection of an Efficient AAV Vector...

  12. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...world of fluorescent proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida...been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists...collection including some 3,300 plasmids along with image galleries and educational resources to go with them...Davidson lab even included some of their own reference images that can be found on the corresponding plasmid ...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...developed dLight1, a molecular tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar...exhibit high (submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed genetically ...

  14. RUBY-Red Siliques

    Type
    Blog Post
    ...sterile conditions where disturbing the tissue for imaging or collection could contaminate it. I have used...self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res... highly expressing RUBY plant is a vibrant red/magenta while (bottom panel, left) a lower expressing RUBY... He has a passion for microscopy and beautiful imaging.   References and Resources References  He, Y....

  15. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...your application. Depending on the tag, directly imaging or tracking your protein may also be possible, ...peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged amino acids...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where...western blot as monoclonal antibodies also exist for imaging. The obvious con of GFP is its size – which can...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...will produce a secondary color (yellow, cyan, or magenta) depending on the hairpin combination. A gRNA containing...the same sgRNA will generate additional colors. Image from Ma et al., 2016 with permission.   Developing...-arm (T1), and the q-arm (T2) of chromosome 19. Image from Ma et al., 2018 with permission.   Why...RNA scaffolds for signal amplification in genome imaging.” Nature Methods 15 (2018): 928-931. PubMed PMID...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General Hospital...that are released from cells during production (Maguire et al., 2012). They saw that these AAV particles...development of the exo-AAV isolation protocol by Maguire and colleagues, other researchers have tried to...: 28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV ...

  20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission. Though...quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. Broadening the spectral...and subsequent transformation into cyanobacteria. Image from Vasudevan et al., 2018. Find the CyanoGate...binding, bringing the two halves of Cre together. Image from Eisenhoffer et al., 2019. This novel tool...
Showing: 121 - 140 of 470 results