We narrowed to 565 results for: mme

...to people outside of academia. Here are my recommendations on some ways to get started sharing your science...others in the science blogosphere is to read and comment on their blogs. 5)   Local science museums are ... research by guest blogging here, just leave a comment if you're interested and our team will get back...

...PITChing MMEJ as an Alternative Route for Gene Editing Michohomology-mediated end joining (MMEJ), also ...favorite CRISPR tool or resource? Let us know in the comments section!...

... hepatic or intestinal cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription...a ‘dead-end' path where cells were partially reprogrammed and re-expressed fibroblast genes. Most cells...cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell gene expression patterns...

...antibodies available at launch were created by James Trimmer, PhD, of the University of California Davis School... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant...

...the CO2 goes out. Conclusions and handling recommendations Not all O-rings function optimally at -80°C...results and the results reported elsewhere, we recommend that once you receive your AAV from Addgene, you...to thaw. However, if this is not possible, we recommend both venting the tube for at least 30 seconds ...

...onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the safranin counterstain...blot gently to dry, and then view under an oil immersion using a light microscope. Gram positive bacteria...a lab.   Anna Kamens is a science communication summer intern at Addgene and a rising third-year at Old...

...are multiple algorithms, both open-access and commercial, that are commonly used for the prediction of...common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate the immunogenicity...doi.org/10.1016/B978-0-12-417144-2.00027-5van Brummelen, E. M., Ros, W., Wolbink, G., Beijnen, J. H., ...

...economically disadvantaged students for paid summer internships. A summer teacher professional development program...with both of these resources road tested over the summer with dozens of teachers around the country. How...

... Roth Lab recommends verifying cre expression prior to use. The lab additionally recommends using an anti-mCherry...effect doesn’t confound your experimental data, we recommend generating a monoclonal Cas9-expression line prior...

...blot out quickly." Final design (right). Immediate and immense regret of the graphic designer not shown...microscopy and flow cytometry experts for the panel recommendations. A few discussions in the review process led...

...conjugates, blocking the conjugation reaction. Many commercial antibodies come with sodium azide already included...reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish...use it for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out...

...but plasmid-based SDM has lagged behind. While commercial kits are available for making small point mutations...plasmid, which could introduce mutations. Using commercially available high-fidelity polymerases such as ...as KOD and Phusion, which are recommended for plasmids up to 25 kb, most cloners should be fine, especially...

...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will.... In addition, authors and readers are able to comment on the referee reports. If the reviewers suggest...

...consensus recommendations, even when a federal mandate encourages them to do so. Immediately after the... with raises, as for years there have been recommendations that postdoc salaries be raised to $50,000 ...

...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving ...Keep it professional at all times Do not post or comment on anything that is not relevant to business or...inappropriate content see this article. However, do comment on other people’s news and updates. Share your ...

...reactions. After all, there is an abundance of commercially available conjugated antibodies that are ready...talking about this? Even though there are so many commercially conjugated antibodies available, that never ...antigen recognition and affinity. Hopefully, a commercially available conjugated antibody will have been...

...consider the reviewer's comments. Whether the proposal is funded or not, the comments help gauge room for ...include for the next application. Although some comments may make it clear that the reviewer misunderstood...

...optimization. Before beginning an experiment, it is recommended that you try a few different ratios of transfer...will need to be optimized prior to starting. We recommend calculating the ratio of the surface area of the... target cell line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing...

...you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG...machine fits on a table top and can be easily programmed for a variety of lab needs. Addgene uses it to... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

...scientists, I have spent hours sifting through comments and papers to find the most reliable (and cost-effective... crisis” came to light as antibodies became commercialized, revealing the unreliability of antibodies....antibody crisis states that up to 50% of all commercially available antibodies are unreliable (Baker, ...