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We narrowed to 568 results for: mme

Showing: 121 - 140 of 568 results

  1. Reflections and Looking Ahead

    Type
    Blog Post
    ...D. W., Kumaran, R., Rego, M., Schofield, C., … Laflamme, C. (2024). A consensus platform for antibody ...10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers,...Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F.,...

  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...comprehensive job description for this co-op, I was immediately interested in applying. This had stood out to...listed with a much less specific overview. It was immediately clear that Addgene wanted to ensure applicants...Letter 7 Tips to Secure a STEAM Internship This Summer  ...

  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

  4. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...to people outside of academia. Here are my recommendations on some ways to get started sharing your science...others in the science blogosphere is to read and comment on their blogs. 5)   Local science museums are ... research by guest blogging here, just leave a comment if you're interested and our team will get back...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...antibodies available at launch were created by James Trimmer, PhD, of the University of California Davis School... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant...

  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ... hepatic or intestinal cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription...a ‘dead-end' path where cells were partially reprogrammed and re-expressed fibroblast genes. Most cells...cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell gene expression patterns...

  8. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the safranin counterstain...blot gently to dry, and then view under an oil immersion using a light microscope. Gram positive bacteria...a lab.   Anna Kamens is a science communication summer intern at Addgene and a rising third-year at Old...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...are multiple algorithms, both open-access and commercial, that are commonly used for the prediction of...common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate the immunogenicity...doi.org/10.1016/B978-0-12-417144-2.00027-5van Brummelen, E. M., Ros, W., Wolbink, G., Beijnen, J. H., ...

  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...the CO2 goes out. Conclusions and handling recommendations Not all O-rings function optimally at -80°C...results and the results reported elsewhere, we recommend that once you receive your AAV from Addgene, you...to thaw. However, if this is not possible, we recommend both venting the tube for at least 30 seconds ...

  11. How Addgene Creates Content

    Type
    Blog Post
    ...blot out quickly." Final design (right). Immediate and immense regret of the graphic designer not shown...microscopy and flow cytometry experts for the panel recommendations. A few discussions in the review process led...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...conjugates, blocking the conjugation reaction. Many commercial antibodies come with sodium azide already included...reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish...use it for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... Roth Lab recommends verifying cre expression prior to use. The lab additionally recommends using an anti-mCherry...effect doesn’t confound your experimental data, we recommend generating a monoclonal Cas9-expression line prior...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...economically disadvantaged students for paid summer internships. A summer teacher professional development program...with both of these resources road tested over the summer with dozens of teachers around the country. How...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...but plasmid-based SDM has lagged behind. While commercial kits are available for making small point mutations...plasmid, which could introduce mutations. Using commercially available high-fidelity polymerases such as ...as KOD and Phusion, which are recommended for plasmids up to 25 kb, most cloners should be fine, especially...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will.... In addition, authors and readers are able to comment on the referee reports. If the reviewers suggest...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving ...Keep it professional at all times Do not post or comment on anything that is not relevant to business or...inappropriate content see this article. However, do comment on other people’s news and updates. Share your ...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    ...reactions. After all, there is an abundance of commercially available conjugated antibodies that are ready...talking about this? Even though there are so many commercially conjugated antibodies available, that never ...antigen recognition and affinity. Hopefully, a commercially available conjugated antibody will have been...

  20. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...consider the reviewer's comments. Whether the proposal is funded or not, the comments help gauge room for ...include for the next application. Although some comments may make it clear that the reviewer misunderstood...
Showing: 121 - 140 of 568 results