We narrowed to 208 results for: pcr

... selection. Screening of transformants by colony PCR confirmed the insertion of the Lambda insert at the...

...plate overnight Screen colonies phenotypically or by PCR/sequencing While CRISPR-transposons are powerful...

...detected directly by Northern blot analysis or RT-PCR. Uses of the minigenome system Minigenome systems...

...genes replaced.  Additionally, 2A peptides could be PCR-cloned between your genes of interest and you can...

... reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize...coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal...background signal that is most evident during initial PCR cycles. This background signal must be removed to...Sing A, Ehrhardt A, Baiker A. Universal real-time PCR for the detection and quantification of adeno-associated...Protocols AAV Titration by qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology...Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis: 30 min Equipment qPCR instrument Heating plate...genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration...

...somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression...

...targeting region from the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA...

...(specific to your target) to screen colonies via PCR. A 354-bp product confirms successful insertion....

... What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial...

...Overall, it worked. We pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would...

... individual gene edited clones are identified by PCR. Then, if necessary for subsequent experiments, the...

...analyzers, and any ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers,...

...vector particle components or performing quantitative PCR titration of vector DNA in samples harvested 24 hours...

...restriction enzyme (RE) recognition site, RE analysis of PCR product could be used to detect success. Although...

...generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein...the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq...depending upon your specific protein folding needs. The pCri system has recently been used to study a matrix ....8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on the system Easing...Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free expression ...

...Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification Resources... use by the Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a ...68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD...

...expressed them along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled samples...

...the tool, you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond... PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers ...

..., SacI restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in...can occur in this system if a small contaminating PCR product formed from primer dimers and containing ...clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed...

... repair templates using Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and ...