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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 210 results for: pcr

Showing: 121 - 140 of 210 results

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...aliquots of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing... hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more...remember to make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws... included. While the physical titer generated by qPCR is useful, I test a few different concentrations...

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize...coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal...background signal that is most evident during initial PCR cycles. This background signal must be removed to...Sing A, Ehrhardt A, Baiker A. Universal real-time PCR for the detection and quantification of adeno-associated...Protocols AAV Titration by qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology...Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis: 30 min Equipment qPCR instrument Heating plate...genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...BsaI none S. pyogenes Kamoun 52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31 rice...

  8. Open Enzyme Collection

    Type
    Collection
    ...this collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein...the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq...depending upon your specific protein folding needs. The pCri system has recently been used to study a matrix ....8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on the system Easing...Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free expression ...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification Resources... use by the Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a ...68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD...
Showing: 121 - 140 of 210 results