We narrowed to 166 results for: pcr

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Get started with Gibson Assembly Cloning!

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

Tips on designing primers for PCR

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

A laboratory research management consultant and coach provides step-by-step advice on how to negotiate a budget when stating your lab.

Protocol for Ligation Independent Cloning

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

Learn about CRISPR plant base editors for plants