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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 407 results for: pho;

Showing: 121 - 140 of 407 results

  1. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...et al. "A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas...Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events...rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the induction of intra...resulting viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas directly ...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...started!   Thanks for all your hard work!   Fluorophores When is a Monomer not a Monomer? The Top Three...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments...experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based machines...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  5. Distribution to Industry

    Type
    Collection
    ...through our website. We cannot accept orders by phone, fax, or email. Pricing Addgene is a nonprofit organization...

  6. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests...have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most...with colleagues and link your notebook from your smartphone to your computer. Lab Archives will help both...

  7. Promega Plasmid Collection

    Type
    Collection
    ...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow...

  8. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...questions. Don't try to get all panelists on the phone at once – you won't get as much out of it and it...better at remembering these things on a relaxed phone call than when first confronted on a stage in front...front so the interactions are better. Have a good microphone system ready for each panelist if the room needs...what happens to the attention of the audience – phones come out, postures shift, they are checking out...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to... coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000). Chromoproteins also enhance... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

  10. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  11. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...size using electrophoresis, typically capillary electrophoresis. Capillary electrophoresis reads a fluorescent...or G) is supplied as a dideoxyribonucleoside triphosphate (ddNTP) so that after its addition, DNA synthesis...

  12. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

  14. Open Enzyme Collection

    Type
    Collection
    ... DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top...

  16. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...trafficking and their detection can suffer from photobleaching and cellular autofluorescence (Tsien, 1998)...plates or media, which can obscure weak signals; photobleaching from the excitation light, causing the signal...generating reactive oxygen species that can lead to phototoxicity, perturb biological processes, and compromise...Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence imaging. Nature Methods...

  17. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...dTomato, displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et ...

  18. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and helper...consistently shown promise in reducing leukemia and lymphoma and are in their clinical trial phase. In all ... this results in frequent physiological and morphological changes. Tumors differ in their genotypic and...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase...
Showing: 121 - 140 of 407 results