We narrowed to 133 results for: primer

1. Special Delivery: Fluorophore Targeting for FRET Studies

   Type

   Blog Post

   ...assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it). Molecular mailbox...

2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   ...ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift...

3. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   ...into the plasmid backbone, and what sequencing primers can be used to verify it. If your plasmid has multiple...

4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...vector (the procedure will simply differ in terms of primer design). Let's assume that your favorite vector...

5. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   ...Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon Read Our Interview...

6. Cloning

   Type

   Guide

   ...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...

7. CRISPR Guide

   Type

   Collection

   ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

8. Plan Your Experiment

   Type

   Guide

   ...qPCR Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...

9. CRISPR Guide

   Type

   Guide

   ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

10. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...encodes a unique restriction enzyme site and unique primer sites for more rapid screening by restriction digest...

11. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...Sequence positive clones with pLKO.1 sequencing primer (5’ CAA GGC TGT TAG AGA GAT AAT TGG A 3’). TIP:...

12. CRISPR 101 eBook...en español

    Type

    Blog Post

    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

13. Intro to Our Summer SciComm Series!

    Type

    Blog Post

    ...absence of a predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only ...