We narrowed to 130 results for: primer

1. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...vector (the procedure will simply differ in terms of primer design). Let's assume that your favorite vector...

2. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   ...Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon Read Our Interview...

3. Cloning

   Type

   Guide

   ...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...

4. Plan Your Experiment

   Type

   Guide

   ... region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...

5. CRISPR Guide

   Type

   Collection

   ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

6. CRISPR Guide

   Type

   Guide

   ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

7. 27 Hot Plasmids from 2016

   Type

   Blog Post

   ...encodes a unique restriction enzyme site and unique primer sites for more rapid screening by restriction digest...

8. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...Sequence positive clones with pLKO.1 sequencing primer (5’ CAA GGC TGT TAG AGA GAT AAT TGG A 3’). TIP:...

9. CRISPR 101 eBook...en español

   Type

   Blog Post

   ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

10. Intro to Our Summer SciComm Series!

    Type

    Blog Post

    ...absence of a predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only ...