...because a process is working, doesn’t mean it will always be that way. Things change and processes often ...intentionally engage in a creative problem-solving process?  You may want to start by carving out time to ...holds the dual purpose of giving people time to process the issue properly while also discouraging everyone...is thrown out because they haven’t had time to process on their own. You may also find your meetings run...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

...powerful resources for investigating any biological process. High quality genome sequence A high-quality sequence...understanding the gene networks that regulate processes from development to metabolism to fertility. Several...mutations in genes affecting diverse biological processes—not just development—are available from strain...Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the zebrafish...and explain how signaling pathways control this process. Additional zebrafish applications and conclusions... providing unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow...

...actually share many biological properties and processes with our own cells. Like human cells, yeast DNA...proteins and tissues to better assess developmental processes. For example, the Stainier lab studied the proliferation...making it ideal to study basic molecular biology processes. C. elegans are also quite amenable to genetic...investigate an array of biological systems and processes including the nervous system, development, cell...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...

...team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job description...leaving the interview hopeful to continue on in the process and I was excited when I received an email to invite...what my position entailed. The official training process was lined out to span the first three months of...the plasmid function. The steps of the training process gave me enough time to become familiar with the...there. At this point, I have seen several lab processes and am currently working on projects for the Science...

... enter the culture medium during subculturing processes or from the air if the cell line remains exposed... infecting mycoplasma. The primers used in the process are either species/gene-specific or universal. .... Some amount of expertise is required in this process because result interpretation might be difficult...by training eligible staff for the cell culture process and hence eliminate one of the causes of contamination...filtration. There is no 100% assurance that this process can avoid contamination but it’s a very good practice...cultures. Elimination is a very time-consuming process and increases the risk of secondary contamination...

...    Figure 1: In the isotype conversion process the variable regions of a parental antibody (blue...isotype (purple, mouse). Depending on the cloning process, all constant regions, just the heavy chain constant...isotype to that of a different group (Fig. 1). This process, called isotope conversion, gives users the flexibility...addition to increasing multiplexing capabilities this process is frequently used to reduce immunogenicity and...therapeutic potential of antibody medicines. In this process, antibodies derived from non-human species like...

...   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python scripts... vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS), to confirm...We recently published a description of the VGS process and use cases in Human Gene Therapy. Existing methods...substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...

...into a plasmid of interest. You can use similar processes to add overhangs to your insert of interest for... The steps following primer design and the PCR process itself are very similar to those outlined in our... with a few quirks specific to the PCR cloning process - please check out that post if you need a more... suited for analyzing the gene’s function. The process is shown graphically in the following figure, in...restriction digestion. As such, the later steps in this process are the same as those discussed in our restriction...

...Allikalt et al., 2020) Impact of labeling process: The process of dye labeling, such as conjugation with...beneficial when studying dynamic processes like mitochondrial dynamics. This process, involving mitochondrial ...specimens has offered illumination on biochemical processes that are crucial in the world of biological research...result in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

...laboratories from using these methods for routine processes like plasmid validation. At seqWell, Inc., our...sample-specific barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...a wide range of input DNA concentrations. This process is explained more fully in the animated Fig. 1 ...samples are brought in line with each other before proceeding to library prep. Even with this “pre-prep” normalization...

...bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed...pneumoniae, while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After ...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...Cas13d CRISPR array processing is robust, with CasRx and either an unprocessed or processed gRNA array (22 ...characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from Eubacterium siraeum (EsCas13d...orthologs described in these papers can independently process their own CRISPR arrays into guide RNAs. crRNA ...

... we had to revamp some of our quality control processes. For the many other COVID-19 related plasmids ... for small batches of samples. As soon as that process is complete, QC scientists prepare Sanger reactions...them inline with our slower, more traditional, QC process. While this has worked well for the early sets ...Ginkgo are striking a balance by modifying certain processes in both core operations. As it has been in much...adapting to a new way of doing things. These new processes have been instrumental in achieving our mission...

... can be used for a diverse array of processes. The food processing industry, for instance, employs psychrophilic...time for a given process. These are extremely useful benefits for any food processing industry as it can...psychrophilic enzymes for milk processing, fruit juice clarification, meat tendering, and oligosaccharide...

...plasmids we distribute through our quality control processes. Every plasmid we receive is rigorously verified...every plasmid we distribute through our deposit process, and then we carefully track samples through our... to find all of the information needed for the process. Always sequence verify Once the physical sample...sequencing If you’re like us, you need a strategy for processing several sequencing reactions in parallel. We ...We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

...plasmid (packaging plasmid). Viral production then proceeds by transfection of all three plasmids into a producer...the host genome as part of the viral infection process (though it can integrate at chromosome 19q13.4 ... via needlestick or cut. Following all sharps procedures for your organization will significantly reduce...best to be well-rested and alert the day of the procedure. Mice move much more quickly than cell cultures...understandably induce some nerves. Following safety procedures and practicing safety culture, however, can let...

...deliverables of your work, such as improving a process or procedure, solving a problem, or making a key decision... industry position is the on-site interviewing process. For both of my academic jobs, I interviewed with...different lab groups without having a formal interview process. Industry interviews, however, were more structured...lasted from half to all of the day.  This whole process may seem foreign and a little overwhelming, but...

...takes a little bit longer in insect cells. Processing Processing of your baculovirus is straightforward —...cell preparations, which we talk about below. Processing The viral supernatant is typically collected ... couple days to increase yield. The collection process is a cycle of collecting the media and adding fresh... fresh media every 12 to 24 hours. Processing the virus-containing supernatant will depend on the virus...

...host’s polymerase, but requires Rep proteins to process a double-stranded intermediate into the single-...media are harvested and purified. In total, this process takes 4-7 days, not counting the time needed to...iodixanol and concentrate the purified virus. This process can be completed in one long day, or the virus ... that was carried over from the AAV production process. Either SYBR green technology or a TaqMan primer...generated from an ITR-containing plasmid. This whole process takes ~3 hours to complete: 1 hours hands-on time...

...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their... become more actively involved in the learning process. As trainees become more comfortable with the open-ended...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...of students active participants in the research process – so if this sounds like an exciting opportunity...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...