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We narrowed to 362 results for: tac

Showing: 121 - 140 of 362 results

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    ... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...solution can be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction...

  4. Pooled Library Amplifications

    Type
    Blog Post
    ...libraries may not have amplification protocols attached.  Final step: validation  Remember, there is ...your library amplification protocol, feel free to contact us at [email protected]. Happy amplifying! More...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...events plays itself out: The researcher breaks eye-contact with any other human in the room. Their eyes are...Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make a model of ...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...PBS (Phosphate Buffered Saline), that comes in contact with the cells should already be at 37 °C. Most.... If they reach confluence, they stop growing (contact inhibition) and it takes them time to recover after...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...the facility that produced the viral vector lot. Contact us to determine which facility produced your viral...experiments were performed on your lot, please contact us. Full sequencing of the Viral Genome Next-generation...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...epitope tags into single FP scaffolds with either intact or darkened chromophores. These probes are both...

  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve as a primer for DNA...continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will keep cutting and repairing...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... write a blog post, edit other blog posts, and contact PIs for an upcoming visit to a university. My to-do... of previous depositors from Boston University Contact Dr. Gilmore directly to see if he knows anyone ...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer...cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing BiPVe3-dTomato...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Theoretically, as long as all the plasmid features remain intact, plasmid multimers function just as well as monomers...multimers? Let us know in the comments below or contact us at [email protected]. Resources and References...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... to mechanism. Do the necessary tools exist to tackle the problem and if so, do you have ways to access... you gave a presentation to a specific group and tack on a few new pieces of data, your talk will probably...
Showing: 121 - 140 of 362 results