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...events plays itself out: The researcher breaks eye-contact with any other human in the room. Their eyes are...Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make a model of ...

...PBS (Phosphate Buffered Saline), that comes in contact with the cells should already be at 37 °C. Most.... If they reach confluence, they stop growing (contact inhibition) and it takes them time to recover after...

...libraries may not have amplification protocols attached.  Final step: validation  Remember, there is ...your library amplification protocol, feel free to contact us at [email protected]. Happy amplifying! More...

...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

...on fluorescent proteins and their applications, tackles much broader issues in this brief volume. He examines... Watsons.” In Part Four, “New Science,” Zimmer tackles more recent examples of scientific advances, and...

...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...epitope tags into single FP scaffolds with either intact or darkened chromophores. These probes are both...

...CRISPR analysis tool is part of Synthego’s full stack Genome Engineering Solutions, which also includes...editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected] if you have any questions...

...the facility that produced the viral vector lot. Contact us to determine which facility produced your viral...experiments were performed on your lot, please contact us. Full sequencing of the Viral Genome Next-generation...

...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target.... If you have specific questions, feel free to contact the Molla Lab (X account: @Kutub_joy). Dr. Kutubuddin...

...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

... write a blog post, edit other blog posts, and contact PIs for an upcoming visit to a university. My to-do... of previous depositors from Boston University Contact Dr. Gilmore directly to see if he knows anyone ...

...the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve as a primer for DNA...continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will keep cutting and repairing...

...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer...cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing BiPVe3-dTomato...

...Theoretically, as long as all the plasmid features remain intact, plasmid multimers function just as well as monomers...multimers? Let us know in the comments below or contact us at [email protected]. Resources and References...

...unedited animals struck me as a “needle in the haystack” type of problem and led to me to explore alternate...lethal mutation should remove much of the unedited “haystack”, facilitating recovery of edited animals. ...

... to mechanism. Do the necessary tools exist to tackle the problem and if so, do you have ways to access... you gave a presentation to a specific group and tack on a few new pieces of data, your talk will probably...

...dsDNA for proper base pairing through minor grove contacts, and thus do not read specific base sequences,... ensure fidelity by interrogating minor groove contacts." Nucleic acids research 32.15 (2004): 4503-4511...

...What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem from...

...CASPR2 Human Mouse IgG2a 177447 Anti-Contactin/F3 [K73/20R] Contactin/F3 Rat Mouse IgG2a 177448 Anti-SK2...