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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 838 results for: tat

Showing: 121 - 140 of 838 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations,...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA ...

  3. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are thus well...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...leading synthetic biologists around the world to facilitate the sharing of parts and information. Addgene...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

  6. Adenovirus Plasmids

    Type
    Collection
    ...modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single...

  7. Distribution to Industry

    Type
    Collection
    ...Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these donor...

  8. Microbiology Resources

    Type
    Collection
    ...solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia virus ORF ...

  9. Worm Expression Resources

    Type
    Collection
    ...Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed...

  10. Viral Vectors

    Type
    Collection
    ...delivery has many advantages for research. Viruses facilitate the delivery of genetic information to hard-to-transfect...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker...

  12. Open Enzyme Collection

    Type
    Collection
    ...Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine ...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...important for tetracycline-dependent repression. Mutating these residues led to the development of a reverse...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...systematic and scalable method to create marker-free mutations, insertions, and deletions at any locus in C. ...

  16. Optogenetics AAV Preps

    Type
    Collection
    ...plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2/other ...

  18. Sequencing Primers

    Type
    Guide
    ...LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer M13 Reverse CAGGAAACAGCTATGAC In lacZ...GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer SP6 ATTTAGGTGACACTATAG SP6 promoter, forward...GPDpro-F CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer GW-3' GCATGATGACCACCGATATG 3' ...pBR322ori-F GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer pBRforBam CTTGGAGCCACTATCGAC In pBR322 ... primer SP6 ATTTAGGTGACACTATAG SP6 promoter, forward primer SV40pA-R GAAATTTGTGATGCTATTGC SV40 polyA, ... primer T7 TAATACGACTCACTATAGGG T7 promoter, forward primer T7 Terminal GCTAGTTATTGCTCAGCGG T7 terminator... early promoter, forward primer LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward...

  19. CRISPR Guide

    Type
    Guide
    ...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be...target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases at the... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting...

  20. Optogenetics Guide

    Type
    Guide
    ...improve these natural opsins - by inducing point mutations to alter the absorption spectrum or adding trafficking...physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic ... variants have been created via genetic point mutations, codon optimization, and chimeric fusion of domains... as light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent...photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/SSFO Step function...function Opsins and Stabilized Step Function Opsins. Mutations at C128 and D156 of CrChR delay the closing of...
Showing: 121 - 140 of 838 results