AAV Purification by Iodixanol Gradient Ultracentrifugation
Type
Protocol
...Reagents Opti-Prep (60% Iodixanol) QuickSeal tubes QuickSeal tube spacers 16 ga needle 18 ga needle 10 mL ... formulation buffer) to top off the tube. Seal the QuickSeal tubes. Centrifuge at 350,000 x g for 90 min... QuickSeal tube with a 16 ga needle and start collecting 0.5 mL to 1 mL fractions per tube. Avoid the ...mL fractions in microcentrifuge tubes. Repeat for each QuickSeal tube. The first 3 mL collected corresponds...phenol red Underlay each solution into a QuickSeal tube in the order below using a 10 mL syringe and a blunt...,000 x g at 18 °C. Carefully take the QuickSeal tubes out of the rotor and place them in a stable rack...a row of roughly 20 open 1.5 mL microcentrifuge tubes in a rack. These will be used to collect fractions...