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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 333 results for: uros

Showing: 121 - 140 of 333 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...think resources like this are why my brand of neuroscience has exploded. Because we have a resource like... visiting Addgene’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed ... recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (Issa, 2023). Time...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...domains. Both systems are easily adaptable to neuroscience applications, including optogenetics, and they...manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed PMID: 26258682...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux...

  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    ...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment...shared by bacteria and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by ...targeting a shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

  14. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol...Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors...

  15. Addgene at SfN23!

    Type
    Blog Post
    ...Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's...Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!   ...

  16. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...

  20. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...CD90.1 selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...
Showing: 121 - 140 of 333 results