We narrowed to 334 results for: uros

...region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine...affected signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to...

...think resources like this are why my brand of neuroscience has exploded. Because we have a resource like... visiting Addgene’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed ... recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported...

...wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (Issa, 2023). Time...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...domains. Both systems are easily adaptable to neuroscience applications, including optogenetics, and they...manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed PMID: 26258682...

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...Rochester Medical Center! Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He ...He recently obtained his Ph.D. in neuroscience from the University of Rochester Medical Center, where he...biology techniques to study inflammation and neurodegeneration. He now spends his time learning and writing...

...Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux...

...slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment...shared by bacteria and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by ...targeting a shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both...

...Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...

...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at...

...lab develops iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described... Dominik Paquet is currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research...

...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol...Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors...

...Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's...Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!   ...

... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

...or His. The Neuroscience Antibody Collection includes over 150 antibodies for neuroscience applications...