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TypeBlog Post...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...
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Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part ... you find these plasmids useful. Good luck, and happy experimenting! References and Resources Additional... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...ranging from 11% to 100%, a level similar to other approaches. Nanoblades can also be used to non-invasively... -
Rabies and Neuronal Tracing
TypeBlog Post...Wickersham et al., 2007). Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...paste, these E. coli are transformed with the appropriate plasmid and dsRNA expression is activated through...phenotypes of RNAi knockdown treatment should be apparent after three dsRNA feedings. Some of the phenotypes... -
Tips for Technical Support Calls
TypeBlog Post..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post.... “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating in this area, ...developing a strategy to distilling a message for the appropriate audience and communicating that message in various... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...