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We narrowed to 961 results for: App;

Showing: 721 - 730 of 961 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...shadows along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

  4. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

  8. Protocol - How to Streak a Plate

    Type
    Protocol
    ...(with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate antibiotic...working near a flame or bunsen burner. Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...
Showing: 721 - 730 of 961 results