We narrowed to 971 results for: lars;
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TypeBlog Post...of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most popular blog post on...
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Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post... of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke ...drug resistance Wood wanted to find out which particular pathways drive resistance to specific drugs in... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post... meeting with someone to ask about a job or a particular position at their company, do NOT call this an... is a good time to ask personal questions about salary, opportunities for advancement, challenges and ... -
Top 10 Open Science Developments of 2013
TypeBlog Post...in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course... -
Pipetting Protocol
TypeProtocol...pipette is an essential tool for nearly anyone in molecular biology. You’ll use a pipette in many scenarios... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt... of and gently in contact with the container (capillary action between the container and the liquid will... -
Transfection for Recombinant Antibodies
TypeProtocol...Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection Protocol...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing plasmids containing... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol... an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...60% iodixanol step Carefully add up to 5 mL of clarified supernatant on top of the gradient. Use 1X PBS... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...strategies for using combinations of AAV-based molecular tools for targeted neuronal manipulation. The ...of interest: Cell-type specific expression of molecular tools and sensors Combinations of classical genetic...