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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 962 results for: App;

Showing: 741 - 750 of 962 results

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...suffer from an inability to easily deliver the appropriate isoform(s) of a given gene, as, many times, the...engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...

  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...from random mutations in the genetic sequence that happen to be beneficial, and persist as a result. Why ...are susceptible to the drug, while the few that happen to possess resistance genes survive and multiply...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...RNAi or CRISPR, non-targeting controls may be appropriate as they allow you to assess the specificity of...us now revisit our experiment. In Figure 1, it appeared as though the shRNA did not knock down expression...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...plasmid-- make sure to maintain this by growing on appropriate media. Note: Purifying your expression plasmid...to make sure your cells have first reached an appropriate density. Cells in the exponential growth phase...

  7. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...add liquid LB to a tube or flask and add the appropriate antibiotic to the correct concentration ( see...Large plasmids usually have a low copy number (approximately one or two copies per cell) and they need to...to grow for longer periods of time (approximately 18-30 hr). On the other hand, smaller plasmids can be...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, ...is an umbrella term for a number of different applications that involve different types of small RNA molecules...

  9. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...adjust this setting as needed. To figure out the appropriate voltages to observe your cells on a flow plot...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

  10. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... cell splitting issue at all if I’d planned appropriately. Back in my undergrad days, I had general goals... do I write things down? It’s more viscerally appealing for me to physically cross things out (it just...
Showing: 741 - 750 of 962 results