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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 961 results for: ARC

Showing: 71 - 80 of 961 results

  1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ... team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed itself ...compatible with optogenetic control. The researchers also showed that Archon1 is functional in a wide range of...activity using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen ...membrane where cell voltage can then be measured. Archon1 is  bright, exhibits good localization, has high...models such as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem...

  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...

  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...into a beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...associated with neuronal activity, such as Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

  5. Genetic Code Expansion

    Type
    Collection
    ...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE experts by subscribing...service was set up in 2022 by the NIH-funded GCE4All Research Center . Browse Synthetase Plasmids The table ...Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-...154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154774 pAS_4xU6-...

  6. COVID-19 Resources

    Type
    Collection
    ...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV-2 Viral...

  7. NETRF

    Type
    Collection
    ...Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research Foundation (NETRF...window) is to fund research to discover cures and more effective treatments for carcinoid, pancreatic, and...Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research Foundation Neuroendocrine...creating and maintaining a highly collaborative NET research community is pivotal in accelerating progress ...materials and resources available to academic researchers around the world. The NETRF is grateful for the... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP...Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol... Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear...surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic...peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome... Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato...Foff 2.0-Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP...dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Boyden 137148 pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP nEF Arch3.3 EYFP Cre and Flp dependent 8 Deisseroth 137149...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws WiChR stGtACR...p2a-EYFP nEF Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR...

  10. Lentiviral Prep Service

    Type
    Collection
    ...) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version... the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially...of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made...direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral particles...direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral particles...
Showing: 71 - 80 of 961 results