Skip to main content
Addgene
Showing: 71 - 80 of 950 results

  1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...into a beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation...

  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...associated with neuronal activity, such as Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

  4. Cre-lox system

    Type
    Collection
    ...Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus Mammalian Luo 47117 pT3TS-Cre...recombination of LoxP flanked DNA sequences. Use the search box below to find the perfect Cre expression construct...construct for your experiment. You can search the table for the promoter, fusion, or expression system...Sauer 11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 ...Ryffel 31132 pCSCre Cre CMV Xenopus Ryffel 31309 pCARCre Cre cardiac actin Xenopus Ryffel 32144 pJFRC170...

  5. Genetic Code Expansion

    Type
    Collection
    ...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE experts by subscribing...service was set up in 2022 by the NIH-funded GCE4All Research Center. Browse Synthetase Plasmids The table below...Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-...154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154774 pAS_4xU6-...

  6. COVID-19 Resources

    Type
    Collection
    ...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV-2 Viral...

  7. NETRF

    Type
    Collection
    ...Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research Foundation (NETRF...window) is to fund research to discover cures and more effective treatments for carcinoid, pancreatic, and...Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research Foundation Neuroendocrine...creating and maintaining a highly collaborative NET research community is pivotal in accelerating progress ...materials and resources available to academic researchers around the world. The NETRF is grateful for the... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP...Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol... Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear...surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic...peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome... Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

  9. Viral Vectors

    Type
    Collection
    ...different types of viruses that are commonly used in research, and to find plasmids that can be used to engineer...engineer viruses for a wide array of research purposes. Ready-to-use viral preparations for select plasmids...virus-mediated delivery has many advantages for research. Viruses facilitate the delivery of genetic information... (left). B) Viral plasmids can be modified for research purposes such that, when delivered to a host, ...but instead encode for a specific gene or other research interest. To produce viruses with alternate (non-virus-producing...giving rise to more virus (as happens in nature), researchers can introduce plasmids to a host to generate ...harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions in...

  10. Optogenetics AAV Preps

    Type
    Collection
    ...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato...Foff 2.0-Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP...dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Boyden 137148 pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP nEF Arch3.3 EYFP Cre and Flp dependent 8 Deisseroth 137149...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC+...p2a-EYFP nEF Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR...
Showing: 71 - 80 of 950 results