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We narrowed to 969 results for: ARC;

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Showing: 71 - 80 of 969 results

  1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...into a beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation...

  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...associated with neuronal activity, such as Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

  4. Genetic Code Expansion

    Type
    Collection
    .... Pairs from methanogens like Methanosarcina barkeri and Methanosarcina mazei are orthogonal both in bacteria...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE experts by subscribing...service was set up in 2022 by the NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that...154763 pAS_FLAG-G1 PylRS G1 PylRS Methanogenic archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-..._4xG1 PylT FLAG-G1 PylRS G1 PylRS Methanogenic archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...FLAG-G1 PylRS Y125A G1 PylRS Y125A Methanogenic archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...

  5. NETRF

    Type
    Collection
    ...Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research Foundation (NETRF...window) is to fund research to discover cures and more effective treatments for carcinoid, pancreatic, and...Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research Foundation Neuroendocrine...creating and maintaining a highly collaborative NET research community is pivotal in accelerating progress ...materials and resources available to academic researchers around the world. The NETRF is grateful for the... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic...

  6. E11 Bio PRISM Collection

    Type
    Collection
    ...well as barcoding with specific enhancers for morphological readout. How to Use PRISM Barcodes are delivered... , a non-profit focused research organization supported by Convergent Research (Link opens in a new window...How to Use Plasmids Additional Resources Protein-barcode Reconstruction by Iterative Staining with Molecular...light microscopy. In brain tissue, these protein barcodes allow for efficient machine-learning segmentation...Sheridan et al. (2025) use plasmids expressing the barcode proteins under the constitutive CAG promoter (Addgene...could also include using individual or subsets of barcode proteins to label and detect multiple injection...bias in packaging representation. To read out the barcodes, each protein tag can be imaged with a corresponding...

  7. Optogenetics AAV Preps

    Type
    Collection
    ...22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG...2.0-Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Karl Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP...Constitutive 8 Roger Tsien 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Edward...Edward Boyden 137148 pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP nEF Arch3.3 EYFP Cre and Flp dependent 8 Karl Deisseroth...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin...CAG ArchT tdTomato Cre dependent 5 Edward Boyden 50972 AAV-SYP1-miniSOG-T2A-mCherry Syn InSynC (non-opsin...p2a-EYFP nEF Arch3.3 EYFP Flp dependent 8 Karl Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a...

  8. Lentiviral Prep Service

    Type
    Collection
    ...) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version... the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially...of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made...direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral particles...direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral particles...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...-AAV1 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...AAV5 pAAV-FLEX-ArchT-tdTomato Optogenetics Ed Boyden AV-5-PV2527 99039-AAV5 pAAV-CamKII-ArchT-GFP (PV2527...-AAV9 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...PV2509 29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446...provide high-quality AAV vectors to the academic research community. The Penn Vector Core produced viral-based...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...

  10. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...window) encourages researchers to assemble and share tools for Parkinson's disease research. This collection...Neurodegeneration Research Collection , which includes other areas of this research topic. Michael J. ...Shared Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools ... Disease Plasmid Resource MJFF Plasmids Shared Research Tools (Link opens in a new window) The Michael...SNCA , and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations...editing tools deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert...
Showing: 71 - 80 of 969 results