...team in the UK is five hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation... team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications with my UK team members...out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when I ...

...working with Addgene since 2014 so I’ve been able to watch the growth since then. What do you think is the ....Paratore: I’ve certainly had a front row seat watching the international growth through my work on the...have two children active in sports, so I enjoy watching their games.  If I’m not at game, I’m either out...

...networker (or if you just aren’t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships... the country with groups who are piloting this. Watch for my upcoming mentoring post in which I describe...naturally out of friendships or work interactions. Watch for the people that are already teaching you and...

...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences...

...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ..., the long-term stability, consistency between batches, and molecular definition of recombinant antibodies...Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What is an Antibody? animation...

... “copies.” This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies,...to non-target proteins), variability (separate batches can perform differently – happens when the antibody...

...activity on some mismatch-containing pairings. During Golden Gate assembly, ligation of mismatched pairs of overhangs...sequence-dependent ligation efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations...

...still virgins. Females begin mating ~6 hours after hatching into their adult phase. It is important to clear...before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after setting up...genetic cross). You can tell when pupae are ready to hatch the next day because the pupae will develop a visible...

...designed to have a perfect match to the reference genome – even single mismatches within a CRISPR guide can...for in the CRISPR guides they used, leading to mismatches between the guides and the lines’ genomes, and...

...does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of a 30 nt target...edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed... targeted nucleotide. Introduction of guanine-mismatches in the guide across from these off-target edits...