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We narrowed to 125 results for: lenti crispr

Showing: 71 - 80 of 125 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...single lentiviral vector provides a powerful tool to modify mammalian cells. Multicolor CRISPR labeling...system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and gene expression...139-42. doi: 10.1038/nbt.3149. Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics...Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create a tool that is quick, versatile...robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the ...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...ng Solid Joung et al. Nat Protoc. 2017 CRISPR Knockout Human Epigenetic Knockout Library ...on the Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control ...

  3. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...exists in vivo. Over the past five years, in vivo CRISPR screens have been used to interrogate T cells, ... Figure 1: Using FITS to perform in vivo CRISPR screens to assess T cell responses and clonal dynamics...Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering the Relationship...cell types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR Lentivirus...Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of.... To date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating...: PMC3844869. 2. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer... Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators: A Practical Guide ... crispr...

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field...delivery Epitope tagging of mammalian genes using CRISPR depends on successful delivery of three reagents...using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA...tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were pre-assembled...csRNP knock-in experiments If your lab is new to CRISPR/Cas9 experiments, there is no need to worry at ...found that 75% of our highest knock-in efficiency CRISPR reagents had crRNAs that bind to the template strand...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are...tropism. Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/...collection of CRISPR libraries . Cellular Barcoding Much like genomic screens, lentiviral vectors are a...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...

  7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert...

  8. CRISPR Guide

    Type
    Collection
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...conducting genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview .... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...

  9. CRISPR References and Information

    Type
    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors... GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...
Showing: 71 - 80 of 125 results