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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: Lif

Showing: 141 - 160 of 469 results

  1. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...Individual Development Plan (IDP) and uses it to match qualified scientists to jobs in industry, academia, and ...find open positions and view their required qualifications and skill sets Taking the time to identify...Communication Networking As the number of qualified scientists entering the workforce increases, EAs...

  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi: 10.7554/eLife.43322. PMID: 30667360; PMCID...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...allowing the device to take on a community-driven life of its own. Nevertheless, there are still many open...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and... B., Marsikova, B. et al. Open microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 ...

  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...evidence (in psychology, cancer biology, pre-clinical life science work, economics) that published research... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...and as the Director of Advising in the Division of Life Sciences at Rutgers University. His dissertation...

  6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...with the desired mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated ...primers, each containing the desired mutations, to amplify both strands of a plasmid template. In addition...pitfall of REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce mutations...

  7. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...is to "domesticate" your fragment: PCR-based amplification can be used to create silent point mutations...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

  9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...DNA damage and instability detection. Cell. Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... I possess.   Bullet points on resumes are like cliff notes, so I use these as a starting place for drafting...technical and transferable skills that make you qualified for the position. Highlight the outcomes or deliverables... have made it to the interview if you weren’t qualified for the position. So prepare for the interview...

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... dozen versions of the toehold switches and amplification primers. This helped make sure that we had a...this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated...global environmental suitability for Zika virus." Elife 5 (2016): e15272. PubMed PMID: 27090089. Resources...

  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...work on the origin of cellular life. His lab has long hypothesized that life started with RNA and a simple...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...

  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...reduced their lifespan. Because these dramatic conditions were experienced in a period of life when the grandparents...statistically significantly associated with reduced life-span, but no changes in the DNA sequence could be...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... industry early would only help. Like any major life decision, the transition felt scary. While I knew...scientific discussion is often part of your daily life. Also, while you’re unlikely to be laid off as an...never-ending cycle of grant-writing that is academic life. Of course, because you will be part of a team in...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...reference sequence and the gene of interest to be amplified in the same reaction. Taqman’s increased sensitivity...of interest Some gene sequences are harder to amplify and quantify than others. If available, ddPCR may...chain reaction methods for the determination of amplification of Plasmodium falciparum multidrug resistance...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    ...exposure under these conditions once the measures were lifted (Servellita et al., 2023).  This anecdote goes ...seven species, designated as letters A–G) exist. The life cycle of AdVs is dependent on modulation of the ... et al., 2003).        Figure 1: The adenovirus life cycle. Image reused from Kremer and Nemerow 2015...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez... timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates HR What if promoting...

  18. Molecular Biology Reference

    Type
    Guide
    ... for the ability of the plasmid to be copied (amplified) by bacteria, which is an important characteristic...sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers can... using bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are... make proteins and other molecules essential for life. Scientists often “sequence DNA” to identify the...pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached to a glass slide... other. These attached DNA templates are then amplified again producing ~1,000 copies of each template...

  19. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...within the scientific community, as they allow for simplified transformation processes, and can be used by ...Gram-negative  Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter...expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife.43322. PMID: 30667360....also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...
Showing: 141 - 160 of 469 results