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We narrowed to 399 results for: MUT

Showing: 141 - 160 of 399 results

  1. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...inactivates the gene and acts as a marker for the mutation. When designing these types of experiments it ...not to use the same resistance cassette for the mutation and for plasmid selection. Additionally, scientists...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating... remove the risk of viral integration causing mutations and gene expression changes elsewhere in the genome...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently...frequently introducing frameshift knockout mutations. Thus, if genetic manipulation one way or the other (knock-in...

  9. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...expression. The regulatory element (wild-type or mutant) is cloned upstream of the luciferase gene in the plasmid...

  10. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...non-functional đť›˝-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    ...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...vector with half the size of the ssAAV genome has a mutation in the terminal resolution site (TRS) to form ...genome with wild-type ITRs at the both ends and mutated ITR at the center of symmetry. After uncoating ...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...gene. One such strain is DB3.1, which contains a mutant version of DNA gyrase (gyrA462) that is resistant... al. “F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic proteins which retain ...

  19. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...additional SapTrap plasmids used for introducing point mutations that was published in a microPublication (Schwartz... E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication 1...

  20. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed...
Showing: 141 - 160 of 399 results