...can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This silent mutation...editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. ... template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https...

...viral and cellular proteins and trafficked to the nucleus. Since the integrase is non-functional and yet ...If you want your cells to express a protein or nucleic acid for only a limited time, IDLVs are ideal. ... off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822...

...end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, dele­tions or other mutations, referred...repair template, such as a sin­gle-stranded oligodeoxynucleotide (ssODN), which contains the intended sequence...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

... cell type, are intermingled in the same brain nucleus: a serotype that works great in a GABA neuron, ...Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016/j.omtn...AAV-Mediated Gene Transfer. Molecular Therapy - Nucleic Acids, 8, 13–25. https://doi.org/10.1016/j.omtn...

...confer specificity of the nuclease; however, many Cas and Cas-like nucleases are promiscuous. In other...effects? The CRISPR-Cas system is an RNA-guided nuclease that either introduces a ssDNA nick, a dsDNA break...

...often terminate with a poly(A). ORF2 encodes endonuclease and reverse transcriptase activities.    ...preferred target site for integration is TA dinucleotides and it leaves behind the CAG DNA footprint from... generated by directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation...

...that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...

...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Currently, the kit only includes two versions of Cas9, nuclease and nickase, but we are now adopting dCas9, dCas9...

...important that the preparation is pure from other nucleic acids (genomic DNA and RNA), proteins and chemical...is the purity. In addition to being free of any nucleic acid and chemical contaminants, these DNA samples...

...variants share the presence of a predicted RuvC nuclease domain, characteristic of CRISPR-Cas enzymes. ...than Cas13 or Cas12 are for the other types of nucleic acids and required a certain sequence specificity...

...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

...2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular...

...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target...

...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...

...adeno-associated virus particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32...

... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...