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We narrowed to 849 results for: HA

Showing: 281 - 320 of 849 results

  1. Adenovirus Guide

    Type
    Guide
    ...response quickly, which has limited their use in therapeutic applications. However, this has also made them the...the human population has been exposed to at least one human AdV serotype and has developed a serotype-...immune response has become a disadvantage for the use of rAdV vectors in gene therapy but has, however, proven... digest patterns to determine that the transgene has been inserted into the adenoviral backbone plasmid...other immune-modulatory molecules. Another strategy has been to use oncolytic rAdV vectors, which are replication-competent... of spontaneous reversion to RCAs, although this has been reduced by providing the E1 gene in trans within... adenovirus (RCA) Mutated adenoviral vector that has the ability to replicate and reproduce within host...

  2. The MTA at Addgene

    Type
    Blog Post
    ...process, though the process has changed a lot over the last twenty years! This has helped us develop a fast... request going forward.  After another scientist has placed a request for your plasmid, they must complete...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...attributes (which turned into www.fpvis.org).  That page has been very useful over the years, and when researchers...system that allows users to see when a given page has unmoderated changes and to view previous versions... online collections of FP spectra. Every protein has a page with all the information in the database relating...

  5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ... dronpa fusions. With these various designs, Xin has been able to develop photoswitchable Intersectin,...Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety... of cells that actively produce the HMOs. Fatima has demonstrated the successful production of both types...

  6. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539 ...121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  ...

  7. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... to the original paper that everyone in your lab has been referencing for the last 10 years, and everything...communication further up the collective priority list. There has been progress—for example, a number of funders (e.g...joined Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...birth several weeks previously. While this claim has yet to be independently verified, the watching world...Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on the social and ethical...considerations, in making sure that the research has been adequately proven to be reasonably safe and ...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...easy. Most of our equipment has been donated by local companies and BosLab has grown into an organization... biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules...

  10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...amplified and none are lost. Each Addgene library has its own depositor transformation and amplification...product for next-generation sequencing Once the DNA has been extracted, the representation of the library... success of your amplification. I hope this post has helped clarify some of the important considerations...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...derived from the same cell type; however, their usage has been limited by technical problems. For instance,...binary systems in which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated...effects. Third, protein purification (once a protocol has been established) may be less laborious than viral...

  12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...sequence if necessary. The example plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert....resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the ...reference point so you can easily assess how far the gel has run. Bonus: The dyes also run at predicted sizes ...

  13. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of...imaging neurons, Griesbeck adds that this color change has a particular advantage for observing events taking...Maarten Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...

  14. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer...prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at about ...

  15. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...genome can occur, although at a low frequency which has been estimated to range from 0.01% to 3% depending... well-characterized and extensively studied, and has a broad tissue tropism. Table 1 gives a summary of...across a broad range of cell types in vivo. AAV-DJ has been optimized further for specific cell and tissue... tropism towards specific cell types of interest has been to create and test capsid libraries with random...systemic capsids. A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous...naturally cross the BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that ...preps . AAV retrograde (AAVrg) Directed evolution has also been employed to engineer an rAAV serotype variant...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    ...off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery to Intended...on-target delivery with various delivery modalities and has the potential to reduce negative side effects. This... assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection... collection since we began distribution and has been verified to perform well in standard IHC and western...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all of...they were prior to amplification. And as anyone who has worked with individual plasmids knows, some plasmids...size and backbone.  Verification Once your library has been amplified, you'll need to verify your library...transduction conditions, you’re ready to transduce! Addgene has a transduction protocol you can follow and a troubleshooting...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... Cas11-like protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature...kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which can “drag and drop” kilobases...PASTE is more specific and efficient than HITI and has higher purity of insertion than both HITI and HDR...therapeutic applications. The potential of CRISPR has yet to fully taken advantage of, but exciting new...

  19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only...SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that further expand the...single A, U, or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as...This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus,...

  21. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic tools...Simplified in vivo delivery using SPH mice CRISPRa has largely been limited to in vitro settings due to ...can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach... or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because CRISPRa...

  22. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic ...on a plate! Antibiotic resistance can arise, and has arisen, for many different reasons. Exposure to antibacterial...presence of a known concentration of antibiotic that has been impregnated into a paper disk, and observing...resistance; it’s a natural process of life and one which has allowed bacteria to evolve and survive for millennia...

  23. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...world, is not inert. In all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize...artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet many investigators...dimerizing protein of interest fused to a dimerizing FP has potential to form large stable polymers of dimers... monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated ...

  24. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe...advantages? Fujii: In the last several years, my lab has been working on development of technologies for biochemical... systems to Addgene in the near future. Addgene: Has your lab been working on any other technologies that...plasmids. The merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred...

  25. Editor's Choice, July 2016

    Type
    Blog Post
    ... to monitor cellular perturbations to how CRISPR has made it much easier to generate mouse models.   ...data from the Kim and Joung Labs showing that Cpf1 has lower off-target editing and similar on-target editing...

  27. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...science and our plasmid collection (what science has been hot this year and how do you creatively turn...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout for...

  28. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

  29. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...

  30. Antibodies 101: Validation

    Type
    Blog Post
    ...Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that...good idea to pause and make sure that your antibody has been appropriately validated so that you can have...application. The goal is to show that your antibody has been validated using at least one of these strategies...is binding to something unexpected. This approach has high specificity, but you need to be aware of any...found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation...

  31. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...partnership was fruitful and a first version of the study has just been uploaded to the preprint repository BioRxiv... platform; while they are a minority, their help has been essential to push certain projects. The idea...year on, our biggest lesson running the platform has been the realization that there is a great untapped...

  32. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...in previous animal studies suggested that fasting has a positive effect on cancer treatment (Ref 1). As... mutations and clinical responses. This platform has the potential to become an open-source pipeline and...of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the...

  33. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...useful research materials and information. Addgene has been successful in helping thousands of sharing scientists...depositors and partners. The Addgene Scientist Team has been developing expertise in answering questions ...from plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative...

  34. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

  35. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...but reproducible success remains elusive.  There has also been a lot of effort in immunomodulation or ...AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms.  ...

  36. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...Detailed figure legend here.     The Trimmer Lab has continued to work to make the sequences of monoclonal...collection and make them publicly available. The lab has also expanded their recombinant affinity reagent ...

  37. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...makes a good mentoring program. If your organization has a mentoring program, consider tapping into it and... have going on locally. Your university probably has an alumni directory. Alumni who enter their information... own peer mentoring circle. This type of program has many advantages and I am seeing a lot of success ...

  38. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...Request, an expansion of our viral vectors service, has officially launched! As of today, you can request... on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the...

  39. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...anytime. The CSV is a rich source of information and has data on the date, material, requesting PI & organization...

  40. General Transfection

    Type
    Protocol
    ...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 µm...
Showing: 281 - 320 of 849 results