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We narrowed to 987 results for: IGH@;

Showing: 281 - 320 of 987 results

  1. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...While I had always enjoyed learning about biology in high school, it wasn’t until I started working in my ...molecular biology-based activities making their way into high school classrooms, like gel electrophoresis to separate...led me to develop BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried, cell-free... that students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes...initially developed the BioBits kit to be used in high school classrooms, but it could be widely adapted... a biology teacher at the elementary, middle, or high school level, feel free to check out one of our ...PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit....

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...commonly used, PVDF tends to be slightly more popular due to its durability and high binding capacity. In addition... (HRP), that emits light upon reacting with a specific substrate. The emitted light, or chemiluminescence... samples, a protein standard of known molecular weight in a separate lane can help confirm the size of...acrylamide content of the gel. For small proteins, use a higher percentage of acrylamide to increase migration ...broad range of protein sizes but takes hours to overnight.  In contrast, a semi-dry transfer only requires...chain. Consequently, antibodies validated for IP might not work for a western and vice versa.  In addition...specific experimental condition you could look for a slight size shift in the protein band before and after...

  3. A Control for All Seasons

    Type
    Blog Post
    ...Antibodies 101: Choosing the Right Isotype Antibodies 101: Selecting the Right Antibody Resources on Addgene.org...groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation. By including...protein standard (P) to check for tau molecular weight shifts. A negative control is a sample or ...runs. If the results vary considerably, then the high degree of variability in the process could make ...signal from your target needs to be significantly higher than the background noise (Waters 2009). If the...the fluorescence from your target is extremely bright, then the noise likely will not interfere. If the...control for all new samples, protocol changes, or when high background labeling occurs (Burry 2011). Complex...

  4. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...junior faculty member at Harvard Medical School/Brigham and Women’s Hospital (BWH). Based on her experience...years ago looking for their career improvement) and bright students that will be the future of the country...to help build, together with the universities, a bright future. IMP provides matched one-on-one international...Although all mentorship relationships are valuable and highly recommended, a mentor with international experience...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of ...representatives from different Spanish universities and high level public and private institutions also showed...website Learn about the Mentor Circle Program at Brigham and Women's Hospital Visit the International Mentoring...

  5. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... be on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia (that...business or follow up. Use the "Contact info" link right below their photo. 2) Personalize all connection...note, you waste an opportunity to start things out right. Also, many people on LinkedIn don’t appreciate ...connect button unless you’re on a profile page. You might be looking at a list of suggested connections and...connect people that want to meet or that you think might be helpful to each other. This is a quick way to... before you’ve had a chance to proof read. This might not be important for a good friend, but if you are...and it’s still connecting with a stranger so is highly discouraged. You should be using your network to...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce...Gutless Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are devoid of viral genes and ...belongs to this category. 3rd Gen AdV (Gutless or high capacity AdV) are devoid of all viral coding sequences...proteins in trans. The Gutless AdV have generated high interest for gene therapy due their increased cloning...more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will become...separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed and stored at -80C. ...account. You're now ready to use your purified, high-titer rAdV in your experiment. Enjoy! References...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...midst of a crisis. The “antibody crisis” came to light as antibodies became commercialized, revealing the...goldilocks antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules...creating a public and accessible database for low-cost, high-quality antibodies for IHC. Working with collaborators...their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than polyclonal antibodies because ...from the negative control, such as showing a very bright signal only in the hippocampus compared to other...the amount of time the sample needs to be under light to discern the signal. If the exposure time is long...allowing scientists to find and utilize reliable, high-quality antibodies. We are creating a database for...

  8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...catalog to help you find the right split-FP plasmid for your experiment.  Highlighted Split-Fluorescent Protein...bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques...amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-...Improved sfCherry3C(1-10) for use with sfCherry2(11) Bright split red fluorescent proteins for the visualization... chromophore formation (abundant in eukaryotes) Bright monomeric near-infrared fluorescent proteins as... with exogenous fluorophores, which can even be bright enough for single-molecule tracking in live cells...

  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...PAM) might work too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding...the Future, and big hair. To what was perhaps a slightly smaller population, something else was also suddenly...conference or a karaoke bar), you probably want to sigh. The problem is that many of these studies report... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...reality, editing your target site is not as straightforward as expected. TET1 can have many effects independent...and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs can be achieved by ...

  10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...for fusion proteins! Despite multiple studies in high profile journal articles, many researchers remain...primarily as a dimer in cells while a protein with a high micromolar or low millimolar Kd is unlikely to form... Following the simple logic outlined above, you might think EGFP is unlikely to form dimers in a cell....then their effective concentration will be much higher than if they are homogenously distributed and mobile...concentration of FPs around that fusion protein is very high. Real world examples of these situations in cells... the POI. These structures are large (microns), bright, non-physiological, and difficult to ignore [1,...normally self-associate into homodimers or even higher order oligomers. Unfortunately, FP dimerization...

  11. Common Injection Routes in Mice

    Type
    Blog Post
    ...injection site is straightforward, as it targets large muscle groups (usually the thigh), though it is important...injection, like you might after getting a vaccine. Restraint: mechanical restraint Location: thigh Gauge: 25–27...use depends on a number of factors. You have to weigh the pros and cons of various methods and choose ...circulation is slower, and more institutional oversight is required. Restraint: mechanical restraint or...such as experimental therapies or pain relief, straight to the stomach. This technique involves administering...Intermediate Intramuscular Mechanical restrainer Thigh 25–27 < 0.02–0.05 mL Beginner Subcutaneous Back...

  12. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...emission spectra, meaning they will emit fluorescent light at similar wavelengths (See this handy emission ...against APC-Cy7, the populations are in more of a straight line and are only positive for APC (Figure 1B)...horizontal line, so our circled population on the right needs to shift towards the X axis to become a perpendicular... plot in 2A, the populations are not perfectly straight, but the center of each population is in a line... sample you are trying to compensate in the top right corner of a flow plot, meaning that it should be..., it will show up as a diagonal line in the top right corner of the plot and large compensation values...positive sample. The cell population in the top right corner of the plot is positive for both markers,...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... completed with the Ig light chains. The variable region of the heavy and light chain determine what antigen...going to spot the whole, long word right off the bat, but you might see a few letters and be able to guess...Two immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region... of an antibody including Fc, Fab, heavy chain, light chain, constant region, variable region   The...made up of the variable regions of the heavy and light chains fused together to form a single protein that.... The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently transferred to...interest and detect its presence. Then, the amount of light that can pass through each well - the optical density...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...and understanding of CRISPR, and perhaps provide insights and ideas for your own research! Classification...categories, arriving at a consensus was not as straightforward as it seems. There is not a single gene that... sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental...cutting up DNA after recruitment by Cascade. As you might imagine, this is handy for creating large genomic...adaptations for increased fidelity, activation/repression, high precision edits, and more. Figure 3: Schematic...Cas12 create short 3’ overhangs, which leads to slightly increased efficiency when conducting edits using...with BioRender.com.   What’s your type? It is highly unlikely that you will have to know and understand...

  15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic frame with...and versatility, the technique is remarkably straightforward to learn and relatively cheap (compared to ...manipulated a number of the opsin genes to alter the light spectrum for activation and the kinetics of opsin...above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation...potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps, activation of which...expression alone is extremely limited. Therefore, highly expressing, generic neuronal promoters such as ...privately communicated to me as one of these extremely high expressing promoters). Conduct a time course experiment...

  16. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...parts of our code, we might be calling something a sample and in other places, we might be calling it an aliquot...soundness of the software design. Each of us brings slightly different cognitive biases as a result of our ...creates trust amongst the team. Whether we are a highly collaborative team because we are using the Scrum...we are using the Scrum framework because we are highly collaborative, we love teamwork. Working collaboratively...parts of the shipping code. The following sprint we might have an EMTA theme and we will all be working in... Most of the time, updating these libraries is straight forward. However, there are times where a library...no further updates, security or otherwise. This might sound like Greek to you, which brings me to my next...

  17. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable ...the lab and by offering advice on areas where you might need some help. Today in our transferable skills...with SnapGene, for example, allows us to display high-quality maps and annotations without us having to...events can be very tough. First I needed to find the right contacts at the companies and then convince the ...Great progress! You are collaborating with your neighboring lab, you've met interesting people at conferences...are the individuals that are part of them. I’m delighted that these collaborations give me opportunities...An Imperative for Graduate Students" from Inside Higher Ed Additional Resources on the Addgene Blog Check...

  18. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...densities that are low enough to prevent confluency but high enough to promote cell growth. Overgrown cultures... age of your culture post thaw. In general, the higher the passage number of a culture the lower the transfection...be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you are unsure of...with pH changes and resource consumption due to the high cell density of the culture. In addition to benefitting...harvest approach is that it tends to result in a higher volume of lower titer virus. In such cases you ...downstream applications. While this ensures that the highest titer virus is used, it is not always feasible,...titered. Most titering approaches transduce into highly permissive cell lines such as HEK293, H0 or A549...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...have limited use in vivo because they are based on light which is very easily scattered by tissue. Ultrasound...modality with good tissue penetration depth as well as high spatial and temporal resolution, making it ideal...vesicle genes from Serratia sp. 39006 produced the highest levels of nonlinear acoustic contrast in E. coli...). Gas vesicle expression in vivo is also straightforward: after the E. coli have colonized tumors, inject...Methods to assess bARGSer expression, from left to right: colony whiteness on inducer plates, white inclusions...We wanted to create second-generation mARGs with higher expression, a smaller genetic footprint, and nonlinear...chromatin-opening agents. It ended up being very straightforward to engineer various cell lines from different...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...and writing are key skills. Other fields, often highly technical or skills-based ones, may not use them...are less likely to require cover letters, while higher-level jobs are more likely to consider cover letters...that asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab ...]. Notice that the intros above allow you to highlight what specific skills, qualities, or interests ...considered cover letters to be more important for higher-level positions. Many did not require cover letters...-neutral greeting like “Dear Hiring Manager.” Highlight your achievements with specific examples. Instead...

  21. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...IBEX is a highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching...bleaching to allow for high-resolution imaging without degrading the tissue (Radtke et al., 2020, 2022). I do... do mean highly multiplexed: you can image over 65 different parameters using this method (see Movie S8..., you’re probably also very interested in having high-quality antibodies and reagents that you know work...you’re certainly not alone – we’re all looking for high-quality antibodies and reagents that work for our...immunolabeling and chemical bleaching method for high-content imaging of diverse tissues. Nature Protocols... Data with Addgene Antibodies 101: Choosing the Right Isotype...

  22. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ... for a reply to an email. You need answers: the right ones, quickly, and this is why we have a dedicated...phone call or write out an email. You need help right at the moment you’re looking for it, or you may ... from your colleagues, to intellectual property rights and handling your constructs once they reach you... new feature on our website; towards the bottom right corner of each page there’s a blue icon displaying...result. By clicking on the result links themselves right from within the widget, you’ll navigate to articles...on the "Leave us a message" button shown to the right, you will be be prompted to enter your name and ...team’s attention, where we can then offer the same high level of direct service you’ve come to expect from...

  23. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...together DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created...assembly work? The required homology between neighboring fragments can be created via PCR amplification...including regions of homology to the vector or neighboring fragment. Then you would amplify the fragments...success rate of this reaction is typically fairly high, so there is usually no need to screen a large amount...structure, such as a hairpin or a stem loop (as might be anticipated to occur within a terminator sequence...required single-stranded annealing and priming of neighboring assembly fragments. Gibson assembly is commonly...vector that you want to use is very large, has a high GC content, contains a lot of repeats--any of which...

  24. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...via gene therapy, so researchers have set their sights on another approach. Since shorter forms of dystrophin...to persist over time, stem cell editing would be highly desirable. Should stem cell editing occur, the ...and AAV9, respectively. Although each study used slightly different methods, they each observed the same..., the relative abundance of exon-skipped mRNA is high, likely because this mRNA is not subject to nonsense-mediated... very low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper ...CRISPR delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, ... rigorously examined over a long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should...

  25. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...transcribe the extremely long, highly structured HCV genome (~9.6 kb of highly structured template!). MarathonRT...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Listen to the Hot Plasmids Podcast!  MarathonRT: A highly processive reverse transcriptase Article contributed...complex heterogeneous RNA samples. Furthermore, such high processivity enzymes and the structural understanding...antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic yet are still just...

  26. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...Syndrome One of those teams is led by Claudio Aguilar (right) at Purdue University and is working to understand...forward. Xeroderma Pigmentosum Hendrik De Buhr (top right) and his colleagues at the Wistar Institute in Philadelphia...condition leaves patients incredibly sensitive to sunlight and prone to developing metastatic melanomas and... and other cancers. Joshua Wang (bottom right), also of the Wistar Institute, says that the lab got interested...Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at Duke who has recently moved...returning to basal conditions normally after periods of high activity. The new work will help them to understand... Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol University’s Ramathibodi Hospital in...

  27. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...copies can be tightly controlled by titrating the transposase to transposon ratios. High ratios of transposase...efficiency of excision with PBx has been shown to be higher than that of the wildtype piggyBac™ transposase...importantly, unlike Cre recombinase, the PBx is highly specific, and has not been shown to cause genomic...specialized knowledge to help researchers identify the right technologies and strategies for their projects. ... 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J. D. Sander.... Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and I. M. Verma, “Functional..., M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation...

  28. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...tricky at best, and downright infuriating at worst. While it is tempting to just dive right in and start injecting...also transduce postsynaptic cells when used at very high titers (Zingg et al., 2017), which can lead to cells... total injection volume. You’ll often hear that “high titer” is required for efficient AAV transduction...the spread of the virus.  A better way to define high titer, then, is this: It’s the optimal number of...inversely related to transduction efficacy: the higher the efficiency, the lower the titer and vice versa...needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong promoter...amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression where...

  29. Science Career Options

    Type
    Blog Post
    ...specific goal, and when you realise that your goal might not lead to a career you actually want, you can ...applicable to a wide range of industries and is a highly desirable quality sought after by employers. These...many employers. Increasingly, computer skills are highly desired, as roles are becoming more reliant on ...Programming skills (Python, R, SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science...give you an idea of what additional training you might need to obtain. You'll also need to think of other...you have the relevant skills and experience.You might not normally think about it, but hobbies and volunteer... job involves traveling, showing that you are a highly organised and experienced traveler by citing specific...

  30. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... in Paris and New York. We want YOU to join the fight against coronavirus We are just at the beginning...be the next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in...that we have to stick together (6 feet apart), to fight the new Coronavirus. To help get you started, here... sites - so cloning projects should be fun and straight-forward. All constructs are tagged with a 2xStrep-tag... gave a lot of background in our study - so you might want to be careful with these clones (and help us... research. For all these reasons, the QCRG is delighted to be partnering with Addgene, who will be taking...bloggers Manon Eckhardt (left) and Melanie Brewer (right) from the University of California, San Francisco...

  31. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...fold increase in brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and ...action potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f...GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant... variant. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging small neuronal...processes (dendrites and axons). jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence...lowering sensor resting fluorescence, while maintaining high peak fluorescence) and is useful for imaging signaling...

  32. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...convince your potential employer that you are the right candidate for the job. How can you incorporate the...you need to have a good idea of what should be highlighted on your resume, and tailor the resume for the...programs to predict protein structures, then you might know a thing or two about computer modeling. Make...transferable skills Some of the skills you developed might not have come from the research itself, but from...universities actually have career services offices. You might think only undergraduates use these services, but... work well with your life and career plan? This might be a tough question to ask now, but the earlier ...

  33. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...standard) and reflects a desire for very high MOI on top of very high coverage. Please see this paper for ...that this will require co-delivery of two AAVs at a high MOI, often resulting in a drop in transduction coverage...particles into the same cells in vivo? A: This will be highly dependent on vector dose. The color diversity in...experiments using PHP.eB in the brain give some insight into codelivery efficiency at different doses. ...liver transduction. While we have not tried it, it might be worth investigating for both Q6 & Q7.For epithelial...that we have in house!), which seems to me a very high dosage. Any thoughts?  A: The question refers to...

  34. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly higher mutation rates...this system is clearly also robust enough to ensure high levels of gRNA cleavage.   Xie et. al constructed...nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either a 1-7 base poly(U)tail in tRNA-gRNA... had biallelic mutations at all five loci. This highly efficient multiplexing further illustrates the ... Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter...

  35. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...plasmid may be expressed at higher than normal physiological levels due to high copy numbers. This creates...isolation, a well-defined genetic system, and a highly versatile DNA transformation system.Unlike most...similar to bacterial plasmids and are considered “high copy”. A fragment from the 2 micron circle (a natural...pombe plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region ...antibiotic selection when working with yeast, due to high rates of spontaneously occurring resistant mutants...in the cell, thus allowing the yeast to maintain higher copy numbers. Additionally, improvements in antibiotic...

  36. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...clone out of 104 to 105 colonies. Pro Tip: Use a high-fidelity Taq polymerase with proof-reading ability... center of the sequence. Since lambda red has a higher recombination frequency when the lagging strand...strands. One of the two oligos will recombine with a higher efficiency than the other and you’ll find out empirically...frequency between 0.1% to 1%, and can be increased to as high as 25-50% by designing oligos that avoid activating...deletion of >20 nucleotides 1 out of 104 to 105 Use a high fidelity Taq polymerase to generate insert ssDNA...these strains, expression of exo, beta and gam is tightly regulated by the endogenous phage promoter pL and... allows for a mobile recombineering system, but tight regulation of expression is required for a successful...

  37. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...by large DNA recognition sites, which makes them highly specific.  Jurassic Park by Michael Crichton was...Jurassic Park (1997, 2001, 2015, and 2018) were highly successful in the box office, indicating the insatiable...increase specificity. This modular approach made ZFNs highly appealing to researchers.  TALENs In 2010, transcription...travels. The movie sees the USS Enterprise crew fight against a genetically engineered superhuman Khan...cellular DNA repair machinery, which can lead to high frequency of indel formation in the genome and are...involves The Rock and his super giant albino gorilla fighting off a monstrous wolf and crocodile, that were ...Editing Trading Card seen in this blog post?Download high resolution versions here.  Many thanks to our guest...

  38. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable ...the lab and by offering advice on areas where you might need some help. Today: Teamwork. My first experience...was I to dispute history? And boy did I make the right decision. If I’d spent my senior year isolating ...colleagues, I can also orchestrate the formation of high-functioning-but-temporary teams that have a specific...providing technical customer service. Team members are highly trained, and though there are necessary redundancies...skills - you just want the necessary skills. You might find that your next paper is going to require help... of more than one person. A selfish part of you might not want another person’s name on your next paper...

  39. Antibodies 101: Validation

    Type
    Blog Post
    ... antibody has arrived, hooray! Before you jump straight into the exciting new experiments that you’ve ... their ability to bind to molecular targets with high affinity and specificity. However, there are countless...validation in different applications. Here, I want to highlight the general suggestions given by the International...your bioinformatics skills (or your friendly neighborhood bioinformatician) to look at RNA expression ... tissues.  From this data, you see that there is high RNA expression in the retina and in parts of the...binding to something unexpected. This approach has high specificity, but you need to be aware of any possible...for immunocapture applications and is amenable to high-throughput analyses, however, it requires access...

  40. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Nye in their lab coats handling glassware with brightly colored hazardous liquids. Working in a lab is...school, med school, industry) Other roles you might see Lab manager: managerial duties ex: order materials...makes them excited to do what they do. I would also highly recommend talking to other undergraduate researchers...choosing a good scientific mentor and choosing the right lab. Remember that your time is valuable! Don’t ...scientists are all just people. I’ve found that in the right environment and with supportive mentors, being open...was a bit of a foreign concept to me when I was in high school, but I’ve learned the value in finding mentors...broadening your experiences! These applications can be highly competitive, so asking for help from mentors and...
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