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We narrowed to 1,019 results for: REN

Showing: 281 - 320 of 1019 results

  1. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...to learn how to adapt to different situations and, in particular, to different styles of leadership. As...must accommodate many different types of people in order to harness their strengths and work effectively...to learn more. Having met many people at that conference, I later became involved with the NPA beyond ...within the committee, I became Vice-Chair when the current Vice-Chair stepped down. When the Chair then stepped... the Committee which I then led for 5 years.  Different leadership styles Being an effective leader requires...requires you to understand how to work with many different types of people both above and below you. During...relationships with these individuals who often had different styles from their predecessors. Changes were implemented...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... or looking to improve your current methods, understanding the differences can help you make an informed... Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002). Preferential transformation...make the virus. Viruses can be produced in many different cell types, spanning both prokaryotic and eukaryotic...However, some viruses can be produced in multiple different cell types, including plants, mammals, and insects... transfer plasmid. The transfer plasmids have different packaging capacities depending on the virus. AAV... which system to use will come down to which differences are most important to you — virus type, cost,...this post has provided some insight on these differences in viral production and will help you decide ...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz... Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson...microscopy coupled with techniques that make tissues transparent are enabling microscopes to visualize the cellular...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the...sample and captured the reflected light using a different objective lens oriented perpendicular to the plane...of clear specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar...Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and R,. Zsigmondy.  (1902) Ann...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ... This preferential tropism for different brain regions is largely due the ability of different serotypes...tissue? What is commonly known is that different serotypes have different tropisms but, as you can see, AAVs...there are marked differences among serotypes in their efficiency at transducing different brain regions....serotypes to infect different cell types. In the CNS, it has been shown that there is variable transduction...skeletal muscles, and the central nervous system. Currently, AAVs are being used to deliver CRISPR/Cas9 but...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors, insertional...

  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...presenting your research at conferences such as SACNAS and ABRCMS. These conferences have a lot of programming...her on Twitter @EleanorWang.     References and resources References Canning EA, LaCosse J, Kroeper KM...new therapies can be developed and applied. The current pandemic serves as a striking example of the applicability... is a supportive environment—it makes all the difference in a lab experience. “Mentorship” was a bit of...interested in doing research and attending research conferences. The Barry Goldwater scholarship is also a national...graduate programs, recruiters will attend these conferences to answer questions and offer application fee...explore! Addgene has an entire page dedicated to different career options from science policy to science ...

  6. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses What's the Best Way to... our blog and website.          References and Resources References Ahlfen SV, Schlumpberger M. Effects...Each of these contaminants will have a slightly different impact on the spectra of a mixed sample.  ...A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios.  Protein contaminants...A260/230 ratio of a DNA sample, its absorbance strength is low compared to other reagents, making it more...concentrations < 10 ng/μL), where absorbance readings are inherently more variable. If you do need to work with dilute... instrument you use and check out some of the references we have provided below. And if you do have trouble...

  7. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...across multiple teams or to stay abreast of current trends in the literature, sampling a breadth of work...One thing many scientists enjoy is discussion of current new and exciting literature. As I transitioned ... had concerns that I may not have time to stay current or enjoy that discussion. Luckily, one needn't ...science, researchers of all varieties must stay current with literature and emerging technologies to remain...meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively discussing...the meeting settles in and people get the time recurrently carved out. This notice typically should include...the same figure by figure organization with a different person responsible for each figure. Others still...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...can't distinguish the different proteins from each other. To distinguish different target proteins in your...target. By using a mix of different antibody-fluorophore combinations for different markers of interest, you...fluorophore. However, these aren't strict rules and in practice the differences in brightness levels are ... fluorophore's brightness refers to the signal strength of the emitted photons detected by the cytometer...a global maximum at around 560 nm, leading to different levels of brightness, depending on the laser light's...floodlight. For fluorophore brightness levels, these differences are much more subtle (think of brightness levels... Even though the two compounds are completely different in nature (eGFP being a protein and FITC an organic...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...more scientists in our leadership, and raising awareness regarding the pitfalls of accepting alternative...outside the scientific community. Vanessa Sung is currently a PhD student studying breast cancer at the Goodman...Over the last few years, there's been a disturbing trend in the number of high profile people denying science...climate change is a hoax. This has culminated in the current President holding and campaigning on many views...Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also studies energy...that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University...fingers of skepticism to defend an agenda that halts current data collection, destroys existing data, and guts...

  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...numerous variants for the different types of opsins available and their differences and uses have been discussed... the different variants [1, 2, 4]. And, of course, the best way to learn about the many different opsin...particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses primarily on ...about this in detail later). But what are the different steps in an optogenetics experiment and what are...Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne... or follow him on Twitter @derekpsimonphd.    References 1. Tye KM, Deisseroth K. Optogenetic investigation... for analyzing the neural circuits of behavior. Trends in cognitive sciences. 2011;15(12):592-600. doi...

  11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...strains of mice. This suggests that a small genetic difference between these two mice might drive their divergent...took a broad approach and searched for genetic difference between the 36 mouse strains that are part of...permissive phenotype, but only one, Ly6a, showed differential protein and RNA expression between the brains...level of CNS PHP.B transduction as their C57BL/6J parent. The second generation (F2) of outbred mice also... if PHP.B required the same receptors that its parental AAV9 relied on for transducing cells: galactose...These results suggested that PHP.B, unlike its parental AAV9, does not require galactose and AAVR to transduce...are not reproduced when the vector is used in different mouse strains. The in vivo selection for PHP.B...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...these tools can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...93,491 viral vector preps with researchers in 45 different countries — and as of 2024, we’ve distributed ...the vectors in Addgene’s catalog are offered in different serotypes. In fact, the 879 viral vectors in our... CRISPR, where we dove fairly deeply into the different types of tools scientists were using/depositing... is less amenable to such analysis, due to its inherent bias: Addgene uses a prioritization process to...service. But why are their request patterns so different? It could be a matter of timing, as jGCaMP7 was...from requestors and depositors, and identifying trends in our own repository data, giving us significant...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    ... new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive editing...optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits with similar...similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient for short...types. DIRECTED is compatible with fusogens from different viral families and can be used to target multiple... enzymes, TurboID and APEX2, are localized to different cellular compartments. They showed APEX2 can use...uses sequential labeling by TurboID and APEX2 at different cellular locations. C) Enrichment of dual-labeled...stably or inducibly express tagged iPAK4 from different promoters, specific time points appear as fluorescent...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles to overcome...However, there are many different forms of immunotherapy that work in different ways. For example, a new...learned much about the causes of cancer and the different avenues that can be used to treat it. For those...latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma ...literally thousands of years to Ancient Greece. Current mesothelioma treatment As with many forms of cancer...catch the disease earlier. New techniques are currently being tested in clinical trials to detect mesothelioma...mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy is bevacizumab (Avastin...

  15. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...in the U.S., whose current salaries are below the new exemption level (the current average postdoc salary...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...data) and therefore the numbers of postdocs at different points on this salary range. We have been able...are doing, however, may face greater challenges. Current salary information that we have been able to find... with relative certainty that just over 40% of current postdocs will have their salaries raised to the...already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard group...laws will have on the postdoc population. In the current funding climate combined with the lack of a sustainable...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses Plasmids 101: Optimizing...quantity.   Figure 1: Plasmid preps fall into different categories based on their relative qualities and... and quantities. Image created in BioRender.com. Transformation Grade DNA (low quality/low quantity...fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep, etc. ...what do we mean by DNA quality? There are a few different issues that can arise during the plasmid purification...left with pure plasmid DNA. Image created in BioRender.com. For the clean-up stage, plasmid purification...applications, the choice is largely a matter of preference.   Which size kit to use?  Obtaining higher quantities...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...by Addgenie Rebecca Hutcheson.   References and resources References Banasik, M. B., & McCray, P. B., ...integrase-deficient viral vector. Created with BioRender.com. What are integrase-deficient lentiviral...then, numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV...of non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. ...vectors is random, and the integration site will be different for each transduced cell, there’s no telling which...to valine at position 64) which is sufficient to render the integrase non-functional without affecting ... that of regular lentiviral vectors. The only difference is that you would use the integrase-deficient...

  18. March for Science

    Type
    Blog Post
    ... science) they come from many different places supporting many different positions. It would be detrimental...cycle begins again. No part of this process is inherently political so why is there a march in Washington... (scientists who hold a doctorate degree, are currently working full-time, and have received a research... and everyone is welcome. Why? There are many different things scientists and non-scientists alike are...not necessarily marching for every cause. The references provided also only provide a snapshot into the...microbial interactions, and science education.       References March for Science Website Locke, John, and Peter...Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers need...

  19. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...  7 ways to strengthen your new relationships Here are some ways to go about strengthening a new relationship...events for one organization or go to the same conferences/meetings over and over to get to know the regulars...often the easiest way to build relationships and strengthen them over time.  Things to avoid when following...starfish shape. You can ask me about the different arms of this starfish sometime, but as of yesterday... was only one other person in my class who had children. When I had my baby, we became closer. She supported...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...come across your contacts over the years in many different ways. This leads me to my last tip: It’s usually...

  20. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...deposits to Addgene.  Insomuch as different journals represent different research areas, target readers,... this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits... would also be great to know how much of that difference in citations is caused by depositing a plasmid...wouldn’t be causal. To control for a number of differences between those papers with Addgene plasmids and..., so our estimates now take into account any differences in citations due to the journal of publication... the same journals! So, is all this remaining difference caused by depositing the related plasmids with...it is still suggestive that we see such a big difference in citations after controlling for journal. And...

  21. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...plasmid cloning, and viruses. References Baltrus, David A., and Karen Guillemin. "Multiple phases of ...unique to the lab. Let’s take a look at these different methods of DNA insertion. Transformation Transformation...Various factors promote natural transformation in different bacteria such as growth phase of the cells (Baltrus...plasmid, the genes on the plasmid can give them different phenotypes. For example, this plasmid encodes ...cell. Once inside, phages can follow one of two different life cycles: lytic or lysogenic. Lytic phages ...a common tool used by scientists to introduce different DNA sequences of interest into a bacterial cell...through cell division. You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use...

  22. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... activate different G proteins, work in different neurons and neuroglia, and have different effects on...internalization of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics...Addgene’s chemogenetics guide.     References and resources References Kroeze WK, Sassano MF, Huang X-P,...These receptors can be activated by an array of different ligands, including hormones, neurotransmitters...is estimated that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002...

  23. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...St Pierre is currently a graduate student at Northeastern School of Nursing and currently works as a registered...low-income communities to educate parents about resources that can help their children thrive academically. Rose...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...your relationships to the person as well as their current sphere of influence. In other words, the longer...the better, and their leadership status in their current field of expertise augments their authoritative... while you make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come ...C. St Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program...

  24. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...international mentoring conference held at the University of Valencia (Spain). At this conference, mentors and mentees...international mentors. Universities from five different geographical locations in Spain were enrolled:...mentees held scheduled monthly progress video conferences and had real-time correspondence via e-mail throughout...Mentorship Program and the International Mentorship Conference In addition to a pre-program self-assessment ... exchanged impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary...community overseas. Official representatives from different Spanish universities and high level public and...their support and hope for the IMP program. The conference also honored IMP Mentors & Mentees with awards...

  25. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... words, they were leaving these meetings with different interpretations of what we discussed and decided...scientists learn how to communicate their work differently based on their audience. How you talk about your...at a poster is going to vary depending on the conference you’re attending and who approaches you. You ...with professionals from other backgrounds at conferences or networking events. And if you’re a wet lab...fellow team member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell...certainly wasn’t the only problematic word. How many different things do you simply refer to as a “tube”? We... they could in fact be interpreted in several different ways depending on the team. When Tony, our corporate...

  26. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...everyone at this specific conference, and I was able to include and exclude different modules for this workshop...something about it. Building a diverse team with different talents and expertise Fortunately, the eLife ambassadors...realized that holding the next one at a scientific conference could potentially enable reproducibility practices...it physically to instruct the workshop at the conference, but because each contributor made the materials...open and modular nature of the workshop content: different parts could be mixed and matched to serve a specific...working with communities, not for them - Another strength of this collaboration is that it is driven by ...the lookout for upcoming workshops at future conferences this year. Workshops are confirmed for the Evolution...

  27. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three distinct colors...orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should...specific for telomeric sequences and showed that different fluorescently labeled dCas9s are efficiently directed...target sequence. They succeeded in labeling two different pairs of chromosomes using gRNAs specific to sequences...specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence must be... green, and red. In contrast, a gRNA with two different hairpins will produce a secondary color cyan, ...sequence (as noted in the table). gRNAs with two different hairpin sequences produce yellow, cyan, or magenta...

  28. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...24, 2025 by Alyssa Shepard.   References and Resources References Beumer, K. J., Trautman, J. K....Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions...double nickase system is that you can express two different chimeric gRNAs with the Cas9 nickase which will... like the wildtype Cas9 does. There are a few references for the double nickase system, including one ...system probably have better specificity. A good reference is Ran et al. (2013). As for target selection...most guides would work, but they sometimes have different efficiency). And it is best if the cut site is...for one well in 24-well plate. A good example/reference is Ran et al. (2013). In the case where you ...

  29. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids to investigate for ...WISH technique. The expression patterns of 11 different planarian genes (all of which are available through...phenotypes of RNAi knockdown treatment should be apparent after three dsRNA feedings. Some of the phenotypes...and if you make new plasmids for investigating different planarian genes, we encourage you to deposit them...researchers can easily investigate in planarians. References 1. Alice Accorsi, Monique M. Williams, Eric J...

  30. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...you can quickly test that part in different contexts and plug different characterized parts into a TU! What...works on genetic code expansion.  References and Resources References Weber E, Engler C, Gruetzner R, Werner... then chances are you have been in the cloning trenches yourself. Modular cloning, or MoClo, is a systematic...be assembled into many combinations. Even with current technologies, it can be extremely hard to predict...nature of MoClo allows users to test a variety of different orientations and components within the system ... a great tool to deliver many combinations of different candidate genes at once to identify the best cocktail...and gene editing that use MoClo, for which many different MoClo kits have already been established. Keep...

  31. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... to run an ELISA in your lab!   References and resources References Boguszewska, K., Szewczuk, M., Urbaniak...Antibodies are used in many different experiments that require scientists to detect proteins in their...enzymatic reaction. Types of ELISA There are four different types of ELISAs, which differ in the way the antigen...antibody and the detection antibody recognize different epitopes on the target protein, so they do not... for which multiple antibodies that recognize different epitopes are available; this type of ELISA is ... are coated with another protein — known as a reference antigen — that can also bind to that antibody....antigen as the other types of ELISAs require two different antibodies. As mentioned above, it can also be...

  32. 6 Tips for Grant Writing

    Type
    Blog Post
    ...collaborate with specialists in different fields that can provide different insight into an area - not just...you are looking to move to a different university, perhaps in a different country, choose where you go...collaborators It’s a good idea to move around to different labs during your career; you learn new techniques...I hope to discover. This section may make the difference in whether you get funding or not; applications... grants too There are actually quite a lot of different pots of money available for researchers, so look...gained in writing small travel grants to attend conferences and a seed funding grant for a proof-of-principal...mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

  33. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic reagent that is commonly ...efficiency by 10-fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and ...be stored in single-use aliquots. Never use a polybrene solution that has been thawed more than once. ...in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has...packaging cells for infection If your transductions aren’t working, you can check if virus is being generated...up-to-date tips for your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate...

  34. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...) signal. While many studies focus on promoter strength as a determinant of gene expression levels, the...these will not be detailed here, but additional references are provided at the end.  Nearly all common bacterial..., and III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ...that each eukaryotic RNA polymerase terminates differently. Polymerase III, for example, relies on a specific...Rho-independent termination found in prokaryotes. This is different than Polymerases I and II, which both require ...factor dependent, Polymerases I and II employ different mechanisms to terminate transcription. Polymerase...and/or use weaker poly(A) signals like BGH.  References Peters JM, Vangeloff AD, Landick R. Bacterial...

  35. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... transposition.  Target site: Different transposons insert at different DNA sequences or target sites....specific locations in the genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA... inserted into new target sites. Created in BioRender.com.   Class II TEs: DNA transposons Class ...a target site duplication (TSD). Created in BioRender.com.   Autonomous vs. non-autonomous TEs You...commonly used in the lab While there are many different types of transposons, DNA transposons are most... vectors.   Let’s take a closer look at three different transposon systems adapted for use as research...(Sandoval-Villegas et al., 2021). Target site preference and host range are important to consider when...

  36. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria.... Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined...plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates downstream...following IPTG induction of conjugation genes and bi-parental mating. Following excision from the B. subtilis...a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain a chromosomal copy...components is transferred to recipient bacteria by bi-parental mating. The ICE integrates into the trnS-leu2 ... of guides, promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi...

  37. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some of the inherent inaccuracies of...focus on gene editing techniques. References and resources References Akhtar, W., De Jong, J., Pindyurin...means we could design hundreds to thousands of different pegRNAs for each desired edit, it simply becomes...many more. Once we had our datasets with all the different features and editing efficiencies, we could train...offline via GitHub. The model provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0... predictive power is sometimes limited across different targets due to variations in chromatin context... Our recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024...

  38. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained...spectral flow cytometry are more alike than they are different, there are a few aspects in which spectral flow...complex mathematical algorithm and single stained reference controls to distinguish one fluorophore from another...   Figure 3: Similarity index across 12 different colors on a three laser spectral flow cytometer...conventional or spectral flow. In spectral flow, reference controls tell the software to look for that exact...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...new panel on a spectral cytometer, you prepare reference controls of both beads and cells for a single ...

  39. PhD Applications After COVID

    Type
    Blog Post
    ...yours does and then use that time! Different platforms have different quirks, so remember to practice on...that requires its students to do rotations in different labs before selecting an advisor, it is always...points and keeping them at-hand, you can quickly reference your questions about the PI’s research or the ...the name of the award you won for your conference presentation. It can help soothe your nerves and make ...promoted during COVID-19, online meetings with current students, faculty, and staff have become a new ...of just the program requirements: by talking to current students about the work environment and social ...using the virtual world to your advantage can strengthen your application and help you decide if a program...

  40. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...subtypes of A–G, which come from different species and will have different compositions of Cascade. Although...through G, with different compositions of the Cascade complex. Created with BioRender.com.   The Cascade...degradative power in the future! References and Resources References Loeff, L., Brouns, S. J., & Joo, ... archaea. Class 1 is further broken down into different types, with Cas3 systems making up type I, the... complex from CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing Cas3...of the Cascade-Cas3 mechanism. Created with BioRender.com.   The reeling mechanism The reeling mechanism...
Showing: 281 - 320 of 1019 results