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We narrowed to 1,006 results for: RING

Showing: 281 - 320 of 1006 results

  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...those entering science communication (myself included) come from a scientific background and bring with... (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience major, I ...first semester in which I had several projects nearing deadline (an animated video depicting nuclear fission...level of career satisfaction indeed.   For many venturing forth from tertiary study, the myriad trajectories... scientific research is, after all, one of the enduring goals of science communication.   The Owheo...improve the world around us has long been known; ensuring all have access to this power and the tools to...

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...technology since its initial applications for genome engineering in 2012.  Many of these advances have stemmed... fold induction at 31X. By using a slit pattern during blue light exposure (470nm), the researchers showed... C-terminal (residues 714-1368, C714) halves, rendering the Cas9 non-functional when split but regaining...strategy which had been engineered from naturally occurring photoactive proteins (i.e. CRY2 and Vivid) - Alexander... details, check out this comprehensive review comparing optogenetic CRISPR techniques (Matony et al., ...Built to Last. In: Methods in Molecular Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA... Bratovič M, Charpentier E, Möglich A (2013) Engineering of temperature- and light-switchable Cas9 variants...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... genome engineering, CRISPR is much more efficient at generating the desired mutant offspring, with 85%...adults mated to produce completely transgenic offspring. Although powerful, this technology is cumbersome... Advantages of using CRISPR for mouse genome engineering From the very start, generating mouse models ...microinjectionist, who has the strategically important job of delivering the payload! Last but not least, when using CRISPR...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Cell 154:1370–1379 . https://doi.org/10.1016...Multiple Genes by CRISPR/Cas-Mediated Genome Engineering. Cell 153:910–918 . https://doi.org/10.1016/j.cell...

  4. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ... visiting scientist is distinct from equipment sharing or a collaboration – it requires re-location of...training so that you yourself can become an expert and bring the knowledge back to your home lab. Alternatively...the conversation. Many researchers are open to sharing knowledge and establishing collaborations, so it...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research...mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor (if ...and rights for anything that could be generated during the visit. If you don’t feel comfortable initiating...travels! Resources Resources on the Addgene blog Fostering communication in remote collaborations Transferable...

  5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... body recognizes a drug as a foreign antigen, triggering a humoral immune response. ADAs can inactivate...inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk of an adverse ...on the immunogenicity of the drug product while ensuring the safety and the efficacy.  Better together ...determining the T-cell epitopes that will likely bring about unwanted immunogenicity. The 3-D models can...Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. In J. Bluemel, S. Korte...10.1634/theoncologist.2016-0061WHO. International monitoring of adverse reactions to drugs: adverse reaction...WHO Collaborating Centre for International Drug Monitoring, Uppsala, Sweden, 1992. Mattei, A. E., Gutierrez...

  6. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... Connie Cepko points out the limited choices in hiring graduate students and postdocs when starting out...counter-productive." He also emphasizes the point to “encourage sharing - in part by having projects ambitious enough that...position, start studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire...Nature Jobs for some quick advice and resources for hiring research staff. And for a more indepth resource...experiments and grant writing. You have to enjoy the mentoring role.” She points out that new PIs may not recognize...student or postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement... interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies...

  7. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...world of academic research can be quite daunting. During my first year of college, I knew I wanted to try... four years after I started college, I will be entering a PhD program to continue my scientific endeavors...met graduate students who enjoy the thrill of acquiring a piece of knowledge before anyone else in the...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired by...biofuel production. There’s a lot out there worth exploring! Being excited about the science allows you to...proactive Rather than applying only to posted job offerings, the best research experiences typically come ... provided me with much-needed emotional support during my journey. When you are hunting for labs to join...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    ...allow authors to avoid many of these issues, by sharing their work publicly and immediately once the manuscript...emerging COVID-19 pandemic quickly and openly. By sharing results faster and more broadly, scientists can...evidence of their productivity that can be shared with hiring and review committees, as opposed to the opaque.... Preprints at Addgene Preprints are all about sharing scientific knowledge faster and more openly — which...open-science organizations, we support scientists in sharing their results and materials with the broader community...in a preprint provides all the same benefits as sharing those plasmids alongside a journal article, but...He also posted two preprints of his own research during his PhD studies.  References and Additional Resources...

  9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ... neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration... neurons at specific firing frequencies. She hopes to identify how in vivo firing frequency of PV neurons...researchers attend a conference of their choice while honoring the late Michael Davidson and Roger Tsien who ...iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal ...modulate protein activity and have important roles during oxidative stress. To monitor this activity, Pedre...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...take a look at a few of these methods below. Engineering gut microbes in situ While the ability to monitor... University developed a method for genetically altering gut microbiome members in their native habitat...reporter gene. The hairpin blocks translation by sequestering the RBS, but binding of the “trigger RNA” (in... Counting cell division in the gut microbiota Monitoring microbial population dynamics in the gut can ...one bright particle that segregates into one cell during cell division. To achieve the agglomeration of ...References Leonard, Sean P., et al. "Genetic engineering of bee gut microbiome bacteria with a toolkit...PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature...

  11. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ... genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering on a genomic...toolbox needed for anyone to begin considering genetic engineering projects in a very big way. In one ...scale could be created. Someone could take on an engineering project including hundreds of genes and regulation...complete toolbox for taking on very large genetic engineering projects. Addgene: How easy is it to do this ...animals make. That will be an outcome of genetic engineering.   Thank you to Chris Voigt for speaking with...

  12. Typing CRISPR Systems

    Type
    Blog Post
    ...2023). Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering. Science...interest in bacterial and archaeal immune systems. During this time, scientists discovered many related immune...keep increasing, as efforts using large-scale clustering and discovery algorithms find new candidates ... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121),...1027. https://doi.org/10.1126/science.aaq0180 Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino..., K. V. (2021). Type III CRISPR-CAS systems: deciphering the most complex prokaryotic immune system. Biochemistry...(2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164...

  13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... while engineering viruses Acr genes can be used as positive selectable markers in engineering viruses...Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies,...dimerize which blocks the PAM recognition site (Harrington et al., 2017). Inhibit crRNA loadingExample: ...domain of Cas9 and prevents target DNA cleavage (Harrington et al., 2017). Figure 1: Anti-CRISPR proteins...436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki... EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-Switches for CRISPR-Cas9. Cell 167:1829-1838...

  14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...population. Outbred strains of mice are useful for comparing dominant and recessive traits and their impact...or treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster...generating knockin mice but because CRISPR/Cas9 engineering favors DNA repair via non-homologous end joining...have errors introduced to the nucleotide sequence during the DNA repair process. The addition of small molecules...to transgenic mice, knockout mice are engineered during the embryonic stem cell stage using one of several...blog posts about using Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more...more about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...

  15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...CRY2, although some studies have shown that this clustering only happens under certain conditions. With...drawback of this method is the stray excitation of neighboring neurons in opsin dense regions. To overcome this...able to demonstrate that while illumination of neighboring cells evokes action potentials (APs) in patched...this operon can serve as a living biosensor for measuring caffeine content in samples. The Barrick lab and...concentrations in several beverages could be estimated by measuring growth of the ΔguaB pDCAF3 strain. Find the decaffeination...placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence intensity at...the authors tracked protein activity while also capturing information about spatial and temporal organization...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, Tsien RY. Measuring calcium...concentrations. This will reduce noise and ensure you are measuring the events-of-interest. Sensors suitable for ER...reporters use a single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength...sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a great FRET...expression and integration within the target cells, ensuring more consistent and reproducible results in calcium...subtypes for efficient expression and downstream monitoring. This targeted approach allows scientists to ...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...manipulating cell activity, especially neuronal firing. What is chemogenetics? Chemogenetics relies on...looking at immediate effects of specific neuron firing are better equipped using fast-acting optogenetic...receptors can be used to alter excitability by lowering or raising the threshold for signaling. The two...for neuronal signaling and causes neuronal burst firing. Other DREADDs, such as the KORD receptor, increase...The Power of the Lock, the Weakness of the Key. Favoring the Pursuit of Specific Conditions Rather than...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

  18. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...guide sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of...structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering, they developed...PAM right where you need it, the odds of this occurring are not in your favor. Thus, there is a need to...coverage in the human genome. SpRY Cas9 has PAM sites covering the majority of the genome! Want to get your...Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant with PAM Flexibility History...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    ...amount of time. Though I now spend far less time answering help emails and phone calls, I feel compelled ...can be a particularly good resource if you are considering using a reagent for “off-label” purposes. When... than you might think. The Addgene scientists answering the phone are smart, but they cannot (and should..., but many bio companies have real scientists answering their technical support questions (besides Addgene...Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’t be experts on every...-up when things get crazy. Take home message Answering customer service questions is a delicate balance...Once You Recieve Them Get Help with the Addgene Ordering Process ...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ..., the vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try...store freshly harvested virus at 4 °C instead of storing it briefly at -80 °C. If troubleshooting, the safest...increased by concentrating a viral stock. Different titering methods can reveal differences in physical versus...result of plasmid integration via recombination during transfection, and does not necessarily indicate... infect the packaging cells. Another method of ensuring viral production is to perform PCR against components...adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID:...Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA into normal human...

  21. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... important consideration when preparing the implant itself, which brings me to the second key point; the...cable to the ferrule implant must be maintained during the entire protocol, otherwise optical stimulation...it to a patch cable connected to the laser and measuring its output with a light meter before and after...that one laser can stimulate only a single animal during the course of an experiment. There are a number..., 6]. These have the obvious advantage of not requiring patch cable connection to the laser, which affords...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  22. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible...so hard that we upended our lives and took this daring risk? What moment changed our whole outlook on ... We both had an applied science bent, so the engineering aspect of this job appealed to us. However, since...thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas about ...molded the whole thing into a marketable product. During this process, we had an idea that survived the ...outside of Washington D.C. and has always enjoyed exploring the outdoors and experiencing nature first-hand...

  23. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...experiments simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity...type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox 5 and 6 (Dlx5/6...“caged” by fusion to specific domains thereby rendering them inactive in the cytoplasm. The authors use... Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of new RFP variants The... length with TZAP Multiplexible plant genome engineering toolkit The Voytas Lab at the University of Minnesota...used for either TALEN or CRISPR -based genome engineering across a variety of plant species. This toolkit...variety of promoters for versatile and robust engineering. These components are provided in Golden Gate...

  24. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... first identified in the late 1960s during research at hot springs in Yellowstone National Park.  In addition...Using these reagents and a series of heating (denaturing) and cooling (annealing) steps Taq polymerase... common for dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification... as real time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires...this fluorescent dye, the concentration of the DNA during the PCR reaction cycles is continuously detected... Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex...

  25. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...significant activity on some mismatch-containing pairings. During Golden Gate assembly, ligation of mismatched... assemblies that enable biosynthetic pathway engineering and optimization. However, current best practices...redesign was guided by both advances in the re-engineering of the original BsaI-HF® Type IIS restriction...efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations, accurate...and a Development Group Leader overseeing a team bringing new products to market, New England Biolabs, Inc...comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining." Nucleic acids research (2018)...

  26. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help us understand...nucleotides 1-10 of the guide RNA, prearranged for base pairing with a complementary DNA strand. These 10 nucleotides...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...mismatches in the next 8-10 bases after perfect pairing in the seed sequence.   Figure 6: Electron...stabilizing the active conformation of the HNH domain, ensuring all binding partners are in place before cleavage...still being uncovered, and different techniques all bring their own advantages—and blind spots—to these questions...better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with...

  27. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...you to our depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR Research...antibodies for use in mice and human brain IHC and sharing the data on the mAb3D atlas website.  The iGluSnFR3...Beroukhim labs to write a post on their paper uncovering ancestral bias in CRISPR guide library design...

  28. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene headquarters every...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one... September 13, 2018 This massive scale reagent sharing led us to calculate how much time we have saved...would have saved 5 million hours through reagent sharing. How much is 5 million hours? A colossal 547 years...plasmids to the repository and for the scientific sharing community (check out the infographic below for ...the past month, we’ve enjoyed seeing scientists sharing their contributions on social media as well the...

  29. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...the host cell. Some of these envelope-receptor pairings are broad, allowing delivery into many cell types...allows these viruses to infect neurons. Besides altering the host tropism of your virus, there are many...reasons when you might pseudotype your virus:  Lowering cytotoxicity. Different envelope proteins have... up less of the virus and is less affected.  Altering sensitivity to serum. Different pseudotyped viruses...the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA. This allows ...v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism of Lentiviral Vectors through Pseudotyping...Quick Guide to All Things Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org...

  30. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...pEcCas/pEcgRNA system. This system works well for engineering single genome edits but the double strand breaks...Cas9, a temperature-sensitive replicon for self-curing, an arabinose inducible lambda-red gene, and an... E. coli B and W strains. The optimized plasmid curing method was much simpler and took only ~32 hours...sites edited. We optimized the strategy for plasmid curing, providing users with a complete editing process...interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system in two...compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine biosynthesis... systems to choose from. Ready for metabolic engineering with improved cargo dedicated to gene (cluster...

  31. Lab to Office Culture Shock

    Type
    Blog Post
    ...well. Making the transition from the bench can be jarring for the unprepared, and unfortunately, there aren...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...sure, but it also eliminates that feeling of floundering one can have when starting in an academic lab...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...organizations like Science from Scientists, which try to bring real scientists into schools to provide students...

  32. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...editing in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018)....et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with Cas9 nickase (nCas9). ...to an adenine base editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful...it with dCas9, and transformed it into E. coli harboring the defective CamR gene. Screening for surviving...colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA*) that satisfactorily.... Kang, Beum-Chang, et al. "Precision genome engineering through adenine base editing in plants." Nature...

  33. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... Career Planning Resources. The key to ensuring success during graduate school and beyond is to live in...transferable skills while working in the lab and by offering advice on areas where you might need some help...on what I have found to be especially beneficial during my own graduate career – and in fact, some of the... a job fair! Similar to a conference, job fairs bring you face-to-face with working professionals, with...the ACS career fair, which are larger meetings offering resources for students seeking employment within...graduate schools will even pay for you to attend!), exploring a job fair, and utilizing your local career development...

  34. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic...Synthetic Biology and Innovation - A shared space for bringing synthetic biology to the UK with foundational ... SynBio. Chapter 3: Synthetic biology as the engineering of biology at the level of DNA using modular,...in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic production...lectures synthetic biology for the Department of Bioengineering. He graduated from Oxford University and then...supervision of Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one...

  35. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...Addgene has done a really good job at figuring out how to operate during the pandemic, but it’s important ... addition of Cicalese to the Board of Directors brings human resources experience and insight to the now... the near future? Right now it’s a matter of maneuvering through the impact of COVID-19. I think Addgene...which raises questions like “How do you start to bring people back to on-site work?” and “How do you start...not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, jewelry...

  36. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... a wide variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs.... sharing... Mary Gearing...

  37. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...you for inspiring us with your breakthrough research and your commitment to scientific sharing. In the... of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check out this...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...continue to improve our services and work to make sharing even easier. We can't wait to see what you put ...

  38. Evolution of Lab Techniques

    Type
    Blog Post
    ...mapping to investigate DNA sequence content by comparing the fragments created by different restriction...breakthrough for determining the order of nucleotides in a string of DNA (7). Sanger sequencing uses modified nucleotides...become obsolete. For instance, the popular genome-engineering tool, CRISPR, may one day make techniques used...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...under $1,000 (10). The future of sequencing should bring efficient, low cost methods for sequencing large... as both time and research progress. These will bring faster, higher throughput, and potentially more ...Andrew Cho, and Ashok B. Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols...

  39. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ... shift toward hiring and promotion practices that focus on reproducibility and ensuring the health of ... at Harvard University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid...of different types of replication in Nosek and Errington 2017 and Schmmidt 2009, but for our purposes we...experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies that ...science Twitter is very active with many scientists sharing their unpublished data. There are blogs - both ...novelty and impact. Journals could also begin requiring reproducibility checklists that at least make ...

  40. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share...applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the dynamics...protein binds to calcium it changes conformation and brings the two fluorescent proteins together thereby increasing... blue-shifted fluorescent protein (donor) and measuring the resultant emission from the red-shifted fluorescent...release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light. To avoid overlap...is available through Addgene (Plasmid # 87127). Pairing NanoLuc® luciferase with fluorescent proteins has...in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, 5023-33. PubMed...
Showing: 281 - 320 of 1006 results