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  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...overhangs to your insert of interest for Gibson assembly. The steps following primer design and the PCR...to ligate our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation ...

  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...make sure to rinse off the surface of the filter membrane with the surrounding solution to ensure excess...excess viral particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry...SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... is important and how it should change how the members of your audience think or act.   To address that...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback...

  8. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post
    ...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

  9. Antibodies 101: ChIP

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    Blog Post
    ...that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies ...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References and Resources...

  10. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

  12. A Quick Guide to a Career in Software Product Management

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    Blog Post
    ...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

  16. dTAG - You're it!

    Type
    Blog Post
    ...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

  17. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    .... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

  21. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

  23. Antibodies 101: Fab Fragments

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    Blog Post
    ...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

  27. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

  28. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

  30. Hot Plasmids - October 2020

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    Blog Post
    ...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

  31. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

  33. Hot Plasmids February 2024

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    Blog Post
    ...variants:   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...

  35. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

  39. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

  40. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

  41. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

  42. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi...

  46. An Introduction to Adenovirus

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    Blog Post
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  47. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  48. Tips for arabidopsis transformation

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    Blog Post
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  49. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  50. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...
Showing: 351 - 400 of 616 results