...RESource (EXPRESs) Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors...

...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified...

... using a dCas9 variant as well. Furthermore, they provide a high-fidelity (HF) variant of the BE3 that...bacteria. The authors were able to engineer a dCas9 variant fused to bacterial enhancer binding protein required...

...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...antibodies Higher non-specific binding; lot-to-lot variability Some recombinant antibodies Not always ...antibodies Higher non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not ...

...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...

... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...

...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

...capture a diverse range of CTCs to cope with the variation in cancer, not just the CTCs with high marker ... types, like stem cells or cells infected with malaria or other parasites. Addgene: Do you have any particular...

...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...

...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...

...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...

...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...