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We narrowed to 395 results for: ARIA;

Showing: 141 - 160 of 395 results
  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level data Low-throughput, inappropriate... Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not...sequencing: a comparison of methodologies for KRAS variant detection." Biotechniques 53.3 (2012): 182-188....
  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...ensures that all screened plasmids contain a gene variant* and is amenable to both high and low throughput... recently developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein... Osaka University have developed two new color variants of their Nano-lantern technology, first developed...are designed to complement the original yellow variant, with all 3 having distinct emission spectra suitable...whereby photons emitted by a Renilla luciferase variant are used to excite a fused fluorescent protein,...
  3. Plasmid Collections

    Type
    Collection
    ...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors...
  4. Optogenetics Plasmid Collection

    Type
    Collection
    ...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified...
  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...
  6. Antibody Production

    Type
    Collection
    ...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...
  7. Retrovirus Plasmids

    Type
    Collection
    ...the Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...
  8. Antibody Plasmid Collection

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    Collection
    ... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...
  9. mTOR Pathway

    Type
    Collection
    ...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...
  10. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...
  11. Resolute Plasmid Collection

    Type
    Collection
    ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...
  12. TALEN Guide

    Type
    Collection
    ...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...
  13. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...
  14. Technical Design of a Western Blot

    Type
    Blog Post
    ... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...antibodies Higher non-specific binding; lot-to-lot variability Some recombinant antibodies Not always ...antibodies Higher non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not ...
  15. Editor's Choice, July 2016

    Type
    Blog Post
    ...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...
  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...
  17. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...
  18. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...
Showing: 141 - 160 of 395 results