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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: ATO

Showing: 141 - 160 of 644 results

  2. Depositor Collections

    Type
    Collection
    ...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt... Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen." Scientific...production of a downstream peptide, the transcription terminators in this series are just the tool you need. The...this portion of the BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP ...characterized in Cambray et al., 2013. These terminators can be used to precisely tune your polycistronic...Kit for some insights on its potential from its creators. We hope you’ll find these kits useful in your...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...construct (about 8 in Brainbow-1.0 mice.) The combinatorial effect of multiple, independent recombination...woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene...plasmids References: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous... Lgr5 stem cells. Snippert HJ, van der Flier LG, Sato T, van Es JH, van den Born M, Kroon-Veenboer C, ...PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc...

  6. Cancer Research Plasmids and Resources

    Type
    Collection
    ...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... Figure 6: Diagram of ovary and oviduct anatomy with the equipment used for i-GONAD. A micropipette...  Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with...Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka. “Easi-CRISPR for creating knock-in and...10.1038/nprot.2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light within a specific range of wavelengths. Excitatory channels allow positively charged ions such as...combine excitation and inhibition by using both excitatory and inhibitory channels activated at different... the opsin.      Figure 2: Diagram of excitatory (ChR2) activation by light showing ions flowing... a single pathway to activate various protein regulators at different points in the pathway. Photoswitches...., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical...

  9. Chemogenetics Plasmids

    Type
    Collection
    ...Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used...optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate...

  10. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Find CRISPR plasmids to study the regulatory networks involved in retinal development....mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...

  11. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot...responses to cancer. The screen recovered known regulators, such as Ptpn2, Pdcd1, and Zc3h12a, demonstrating...Moreover, the screen identified putative novel regulators of CD8+ T cell responses in tumors, demonstrating...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...makes time for you, as needed, this is a strong indicator that this person cares about your science and ...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...customized to control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... frequently been used for screening candidate regulatory elements such as enhancers and promoters for ... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....., … Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(...

  14. Distribution to Industry

    Type
    Collection
    ...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...

  15. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...

  17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive measures...Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is ...a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal precision...

  18. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...available to counteract the toxin. If, however, an investigator were to successfully clone an insert into the...insertion will completely replace ccdB with the investigator’s insert of interest. Hence correct clones are... behind the “swapping out” of ccdB with the investigator’s gene of interest.   CcdB resistant E. ...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...activation of keratinocytes, which release inflammatory mediators that trigger the activation of dendritic...Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6...IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both respond to and release...
Showing: 141 - 160 of 644 results