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We narrowed to 570 results for: Cit

Showing: 141 - 160 of 570 results

  1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...screen, it is necessary to identify the gRNAs that elicit the phenotype, and this depends on the method used...fluorescence changes or a requirement for very high excitation light intensities have made these indicators ...surveyed a range of microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis elegans. ... as the eFRET sensors, QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials...

  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...you will find our fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory...over twenty members and volunteers. Transforming citizens into scientists Anyone can become a member of ...microbiome project. This project was inspired by previous city-wide genomic studies and our study will involve ... page on DIYBio.org to find a community lab in a city near you. To learn more about what we’re up to or...

  3. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...its mission to make research easier. Addgene is excited to announce that we’ve added John G. Doench, PhD...at like 7 am to go check your plates and you’re excited to do so, you know that that’s the thing for you...on to apply to graduate school at MIT. Doench excitedly remembers: “When I came to MIT to interview, part...miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This early research...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...diversity in fluorescence brightness, intensity, excitation and emission spectra. The lab of Dimitri Deheyn...group to perform independent two-color optical excitation of neurons. The paper describes five previously...ShChR, nicknamed Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins...method to rapidly address questions of tissue-specificity and cell autonomy of gene function in development...Guo et al., Development. 2014..) A new, higher specificity genome editing system that combines TALENs and... Golden Gate reaction. While the DNA-binding specificity is imparted by the RVD at residues 12 and 13 ...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. But these studies...the genome, but Cas13s have higher on-target specificity. New work from Konermann et al. and Yan et al... 2.8 kb in size and are easy to package in low-capacity vectors! These small, but mighty type VI-D enzymes...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...haven’t been characterized? Some have developed exciting computational algorithms, such as SIFT or PolyPhen...publication We also wrote an extensive review on the exciting new field we call “precision functional genomics....002. We hope that by releasing these new and exciting plasmid collections (together with the data generated...Resources on the Addgene Blog Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage ...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...antibodies that are all of the same isotype and specificity. In other words, monoclonal antibodies are derived...also present in other proteins. Because of the specificity of monoclonal antibodies, they also lead to more... - the paratopes - may change and impact the specificity and avidity of the antibodies produced by the...light chain genes within a hybridoma impacts the specificity, avidity, and number of different antibodies ...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity of attenuated, nef-deleted HIV-1 strains... addition to antibody response 2) very high immunogenicity 3) and long-lasting immune response even after... gene therapy. The tractability of AAV, low immunogenicity, and cell-specific targeting features make ...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cells and have an ~8kb packaging capacity. With this packaging capacity, reprogramming factors can be delivered...other delivery methods. Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic vector...and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral vectors. This ...a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is transfected into cells...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made ...Why would you want to give it a go? The high specificity of Cas14 makes it a promising detection tool ...are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Pharmacology group at Novartis Oncology, is an exciting new tool that allows researchers to individually...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual... and sharing protocols one by one,” and she is excited to deposit the library with Addgene because it ...these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put the ClonTracer...

  12. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...requires only 2-3 hours incubation. E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes..., the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae, without...been observed with Phagemids (Selle et al., 2020, Citorik et al., 2015, Bikard et al., 2014). When the CRISPR... on the presence of specific target sequences (Citorik et al., 2014). These could be genes, specific polymorphisms...7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific antimicrobials...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...on-target editing efficiency. To further improve specificity, they purified HF-BE3 protein for delivery in...conduct comprehensive genome-wide studies of ABE specificity, their other experiments suggest that ABEs are...improved base editor targeting flexibility and specificity. In this first, the Liu lab used phage-assisted...to ABE7.10. ABE8s have an improved base editing capacity, even at sites previously difficult to target..... B., & Liu, D. R. (2017). Improving the DNA specificity and applicability of base editing through protein...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...OpenCourseware for course materials and assignments.  9) Citizen science projects Lastly, if you'd like to directly...public in your research projects, you can set up a citizen science project. Answering the big science questions...downloadable resources to enable you to set up your own citizen science project. Science communication opens doors...way of living out my creative side. My newest, exciting endeavor is starting to write a book.  Many ...

  15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...quantitatively measure protein concentration or antibody specificity via a colorimetric reaction. Image from Boguszewska...quantitatively measure protein concentration or antibody specificity via a colorimetric reaction. Image from Boguszewska...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...contain a single clone they have higher lot to lot specificity and tend towards more reproducible results. Since...work in a variety of applications. The desired specificity of your secondary antibody depends on whether...monoclonal antibodies have higher affinity and specificity than mouse monoclonal antibodies. Antibodies ...filters out off-target antibodies increasing specificity and reducing cross-reactivity. For example, if...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...but also displaying increased editing specificity. We’re excited to learn more about xCas9 - here’s what...editing, to more loci. Although these early data are exciting, there’s a lot we still don’t know about xCas9...variants with broad PAM compatibility and high DNA specificity." Nature (2018) doi: 10.1038/nature26155 PubMed...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...using AAV is the limited packaging capacity. Lentiviruses have capacities of 8–12 kb, while AAV only has ...libraries available at Addgene. The large packaging capacity and stable expression of lentiviral vectors make...Additionally, while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...different packaging capacities depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus... lentivirus has a capacity of 8–10 kb. Time Viral production in mammalian cells is relatively quick. After...transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect cell production...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... abandoned early on due to its considerable immunogenicity and its transient expression. LV results in...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...to cause any disease in humans, and its low immunogenicity is critical when systemic delivery of high ...and safer. It is now one of the most active and exciting areas of science. This is hopefully only the beginning...
Showing: 141 - 160 of 570 results