...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...the reach and impact of your work. Please email deposit@addgene.org if you would like to contribute to ...University of California Davis School of Medicine. This deposit includes frequently cited antibodies for use in...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you...

...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...While the Nuffield Council has recently published a report on the social and ethical issues of genome editing...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...diseases. The key success of the Nuffield Council’s report is that when it became clear that this assistive...

...look back at some of the contributions from our depositors. Below are the 15 top requested plasmids over...2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and contains an envelope... the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE -... Thank you! We would like to thank all of our depositors and requestors for making their reagents available...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids... In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed evolution...

... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find ...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition....org Check out our microbiology resource page Deposit microbiology plasmids with Addgene Find synthetic...

...getting support from depositors. We regularly hear how much they see the value in depositing and they are increasingly... own labs get excited about making their first deposit to Addgene. We are also lauded by another set...large community of scientists who support us by depositing, requesting and spreading the word. Our blog...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for...

...the source or were deposited when the now-published article was a preprint. Depositing plasmids that were... great way to do that. We are happy to receive deposits of plasmids that have been described in a preprint... authors can include the Addgene ID for their deposited plasmids in the final journal publication or an...

...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression... genes (all of which are available through the deposit) are described in the Sánchez lab publication [...different planarian genes, we encourage you to deposit them back with Addgene and expand the number of...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids...future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic ...

...scientist. New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...your own Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...Arokiaraj (Gradinaru lab) Another useful viral tool deposited by the Gradinaru lab, AAV1-X1, was developed by...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's what...2024 under a CC-BY 4.0 license.  The team deposited an array of constructs for tagging the kinesins...Hao and Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...