...result can leave scientists under-informed with respect to how different serotypes, promoters, and injection...conducted to improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data...flaws or gaps in their technical ability. But, especially for AAV data, that may not be the case. We can...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(...

... Although we are busy with many other work responsibilities, we make team building activities a priority...don’t make things too complicated for yourself (especially if you are the sole event planner)! If you have...wise lady for many reasons, but this saying is especially true. If your event requires a lot of set up ...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events...

...LC) is known to play a major role in the stress response circuit by integrating neuronal signalling and...activated, norepinephrine release causes a freezing response, a form of immobility caused by stress and fear...surface. The latter reflects a state of behavioral despair, and the more time the animal spends in the passive...subset of excitatory neurons (Drd1) in mPFC is responsible for the antidepressant effect. To specifically...

...synthesized enzyme. Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein...biotinylated. In E.coli for example, the BPL, BirA, is responsible for biotinylating only one lysine in the entire...Esmond E. Snell was able to purify the protein responsible for binding biotin, avidin. Avidin was aptly ...your protein of interest or primary antibody respectively. Avidin bound to a reporter is then added to...

...differences in physiology, intelligence, and human correspondence that give them an edge as a disease mode (Iannaccone...also easier to study and at times more closely corresponds with human diseases and conditions. For example...scientific history, highly characterized genome, and widespread utilization, Arabidopsis has made significant...Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development. Arabidopsis...

..... Ownership: Misunderstandings about who is responsible for what tasks or projects is a common source...customer support element amongst their other responsibilities. Knowing which aspects of customer support... require a manager to explicitly assign the responsibility in question to a specific person or team. ...one of your strengths. Empathizing with people, especially people with whom you strongly disagree, is a ...

...species on a single sample, you can then use the corresponding secondary antibodies conjugated to distinct ...), and will share some of their results and corresponding protocols. Other researchers may also have tested...Reviewing available antibodies can seem overwhelming, especially when there are dozens of products that all claim... find an antibody in which you are confident, especially if you work in an organism that is underrepresented...

...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...used to drive expression of small RNAs in their respective species. Addgene carries >30 “empty gRNA” backbones...activate or repress target genes in plant cells, respectively. Cas9 expression is commonly driven by plant-derived...excellent alternative genome editing technologies especially in human cells. In contrast to homology directed...

...very rapidly in response to a single light pulse whereas others have slower responses (Gunaydin et al....different opsin (natural and engineered proteins responsive to light in combination with retinal) variations... along a pathway, there can be very different responses to long vs. short stimulation times or frequencies...

... function. When a select group of people are responsible for a group of specific processes, they must ...tasks done. At Addgene, the Scientist Team is responsible for guiding new deposits into the repository,... not someone (or someones) taking on these responsibilities on a team to which you belong, consider stepping...roles they’re meant to fill and what their responsibilities are. That’s a subtle but important distinction...

...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...expression (up to 9 months in mice [2]). Low Immune Response: Compared to other viral vectors (e.g. Lentivirus...Adenovirus) AAVs cause the least amount of immune response. Any investigator who is using an in vivo animal...model should consider the possible elicited immune response from viral infection. Animal safety is a top priority...

...sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and the desired...generation of replication competent lentiviruses (RCL) especially if you are working on HIV positive human cell...generating RCL is low but extra care has to be taken especially if you are working with proto-oncogenes or with...the Tet-on system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter...

...life-saving research are being proposed.  It's our responsibility to join the public conversation and to tell...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed...towards starting these conversations; it is our responsibility to continue the dialogue. Erin Golden is currently...continue this work by voting for candidates that respect science, learning to communicate their science ...

...cancer are often time consuming and expensive, especially when done in vivo. There are also sensitivity...Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics data, and large-scale...mutation or gene fusion. Plasmids carrying the corresponding wild-type genes were constructed as well. They...

...factor in maintaining the group buy-in and morale. Especially when your collaboration includes scientists working...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

...interest and curiosity to understand my work and me, especially as much of her focus is on the scientific work...been done at Addgene before. This meant I was responsible for establishing a new report and routine across..., and make the occasional TikTok. I was also responsible for typical entry level tasks such as stuffing...

...single-wavelength sensors with intensities that change in response to calcium binding. There are a few versions of...endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its F102G variant...with optogenetic activators and inhibitors that respond to blue light. We recently released a constitutive...

...that can extend and retract over many microns in response to pH changes without the need for ATP or other...In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent... neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from Connie Cepko’s Lab at...

... career in neuroscience—whatever that entails. Despite being engrossed by the theory and findings of brain...instrument designed to measure how individuals respond to issues of unsettled science. Understanding just...to understand just how we receive, process and respond to that information. Alex Gilbert is a PhD student...

...major advantage of working with a mentor.The responsibility of arranging meetings and setting the direction...informal minutes on what was covered and learned, especially noting action items that are identified. Keep...checks. Focus on implementation of your goals and respect the time your Mentor is giving by following up ...