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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 804 results for: Eng

Showing: 141 - 160 of 804 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components have ...

  2. Distribution to Industry

    Type
    Collection
    ... Davidson collection Protein Labeling from New England Biolabs Cancer Research from Sinai Health System...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...

  6. Lentivirus Plasmids

    Type
    Collection
    ... a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit in the extended...

  8. The Pleiades Promoter Project

    Type
    Collection
    ...Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...2014. PubMed PMID: 24151867 Mikkelson, et al. Metab Eng. 2012. PubMed PMID: 2232647   SapTrap, a toolkit...genome engineering across multiple species Recent discoveries in the fields of genome engineering and synthetic...pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3...fold a protein because of its length. Mark Howarth’s lab recently engineered a new peptide/protein pair,...as not only a protein engineering tool, but also as a way to characterize/engineer promoters, enhancers... developed for the study of enhancer function, strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...

  12. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG subclass from...expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens in a new...

  13. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...ways of delivering healthcare. Clients typically engage consultants as short-term experts to work on “cases...Cases present specific operational or commercial challenges or questions that clients face imminently. Consulting...on analyzing the situation that presented the challenge at hand and help resolve it through well-informed...specialize in business strategy tend to be mostly engaged by pharma and biotech clients. In this realm, there... there are a few different kinds of challenges that consultants get called upon to tackle, depending on...to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...rewarding parts of the job is being confronted with challenges that are crucial for the success of a drug’s ...

  14. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...accomplish is to make the engineering of biology much easier. So you could imagine engineering the entirety of ...dig into its engineering qualities, what these parts allow is for someone to forward engineer a system of...Drew Endy's lab at Stanford develops engineered DNA systems capable of storing data and computing inside...an organization working to advance biological engineering openly so as to benefit all people and the planet... to not assert property rights for particular engineered genetic sequences; but it’s only the promises... what we think of as an operating system for engineering an entire cell. Typically when people use plasmids...of a cell. That's a big long-term technical challenge. We have a strategy that organizes how we start the...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...in more genome engineering technologies? Browse others on Addgene's Genome Engineering Guide. Fire ...gene targeting Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that can be used to...biochemical characterization, has enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence...transcriptomes, as well as further optimization through engineering, Ed Boyden’s lab has identified a variety of ...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate...field of optogenetics. Using a rational protein engineering approach, they designed Opto-RTKs, which activate... has now made improvements to this system by engineering cells that lack release factor one (RF-1; Bacterial...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher in energy...while photons of higher wavelengths are lower in energy. As such, wavelengths below 380 nm lie within ...can do so at a range of wavelengths, rather than at just one defined wavelength. Likewise, the emitted ...within the UV spectrum, while wavelengths over 700 nm make up the infrared (IR) spectrum. When photons are ...energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher than those of absorbed...emitted photons also appear within a range of wavelengths. For that reason, we speak of excitation and emission...APC-Cy7. Conjugating Cy7 to APC results in a higher-wavelength emission spectrum compared to APC. This happens...

  17. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...conversion tract length in Drosophila. Elliot et al. (1998) suggests mammalian cells' tract lengths seem much...up with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists... the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of ...wildtype or double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase...nickase type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized...Zhang lab has found that if a CRISPR is used to engineer a locus, isolate single cell colonies, then genotype...insertions by Homologous Recombination (HR), what is the length of each homology arm needed? How should the double...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...: A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous administration... post, we’ll discuss how systemic capsids are engineered, as well as highlight some considerations for...region-specific transgene expression. (a) Several engineered capsids with unique tropisms are now available... rodents and non-human primates (NHPs). These engineered capsids have increased transduction efficiency...generally compared to the AAV9 serotype or an engineered precursor of the systemic capsid in question ...better efficacy than AAV9 and/or its intermediate engineered precursor. Considering systemic capsids for your...determined by the capsid and promoter/enhancer strengths in the cell type of interest as well as the type...

  19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder...conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal...Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et...producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. Many split-FP...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang mNeonGreen3 mNG3K...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...ratio. Kodama Y, Hu CD. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments of ...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... specific wavelengths of light to initiate signaling. When FRET donor excitation wavelengths overlap with...proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often use bioluminescence...traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require external...fluor then emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al....that could be co-imaged with GFP using the same wavelength for excitation. The result was the cyan-excitable...This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce a series of FP-RLuc8...concentrations of biological molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in ...
Showing: 141 - 160 of 804 results