... editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency...CRISPR in C. elegans The Meyer and Mello labs also identified several experimental set ups that could improve...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi...systems, allowing rapid progress in a wide range of fields.   Thank you to our guest blogger! Jordan Ward...

...many science policy professionals that entered the field from a more traditional policy background, learning...groups, attend webinars, and learn more about the field.  Read the CCST Science Policy Career Guide to learn...   Where can you do science policy? Because the field of science policy is so diverse, you can do it in...once selected, you can pivot to a completely new field without issue. This may be more challenging if you...you are trying to apply for an outright job in a field where you have no specific scientific credentials...

... cell lines used in scientific research are misidentified! Rigor and reproducibility of scientific data...and collections. Importantly, Cellosaurus also identifies problematic cell lines: ones either suspected...or easily contaminated, as well as commonly misidentified lines. Browsing this feature to know if your... care about. If you have cell lines without a certified origin (you know, that split you got from the ...

... process, the insert and vector are first PCR amplified. The forward primer used to amplify the vector...PCR, you should confirm that the products were amplified by running a small aliquot of the reaction on ...2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent... Our Special Collections for Plasmids from Your Field ...

...technology to survive the diverse demands of the biotech field, let's look at how prokaryotes originally utilized...generate RNA, which guide a Cas nuclease to the specified exogenous genetic material next to the species-specific...re-identifying it (3). Surprisingly, few genes have been identified that neutralize CRISPR/Cas. Earlier this year...lab protocols, providing tips from experts in the field, and enabling access to multiple plasmids used for...

... region. The variable region acts as a unique identifier for individual cells. Each cell is labeled with.... The fixed region acts as a library-specific identifier and allows cells to be tagged with different ...during reprogramming. These library-specific identifiers act like a timestamp and help demultiplex the...this lineage tracing experiment, the Morris lab identified a novel gene, Mettl7a1, whose expression helped...

...small molecules since the very beginnings of the field. Those engineered sensors were designed to replace...1980s. “Synthetic dyes were the standard in the field, but there is one problem: how to get that into ... sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance energy transfer...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

...pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-hSyn-mCherry 50459...for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See our full biosensor... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825...

...selectable marker, and target gene, for example, are amplified with overlapping regions of homology and inserted...for transformation into yeast that same day.  The field of synthetic biology has led to advances in biofuel... in these methodologies offer streamlined and simplified alternatives to traditional cloning. NEBuilder...and opens the door for new opportunities in the field.  Gibson Assembly® is a registered trademark of ...

...conformational changes. These tiny movements are then amplified tens or hundreds of thousands of times by propagating...membranes outside of their natural context or 3) be modified to extend at different pH levels. Domesticating...soluble when extended (see figure to the right), we identified R-body mutants that extend at both lower and ...response to pH changes. Beyond these applications, purified R bodies could also perform as actuators in microelectromechanical...

...emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins in the cephalochordate...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176-81. Zakeri...optimization through engineering, Ed Boyden’s lab has identified a variety of new channelrhodopsins for use as...for these effects, drawing on the rapidly growing field of optogenetics. Using a rational protein engineering...on the Golden Gate cloning method, but has been modified specifically to improve plant transgenesis. TheGreenGate...

... use of microscopy in open science. But the new field of open-source microscopy seeks to change this. ...commercially available consumer-grade components. In this field, often referred to as 'frugal science', existing...Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy, where image processing algorithms...Nevertheless, there are still many open questions to be clarified, especially with regard to licensing models. Direct...

...focused on current questions and experiments in the field of synthetic biology. Founded as a nonprofit in...biology has grown into a recognized name in the field of STEM education worldwide. The BioBuilder curriculum... as a program focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific...module allows students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses...

...the exciting new field we call “precision functional genomics.” The goal of this field is to functionally...correlations between variants in a gene of poorly classified function and a well established cellular signaling...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

...Scientific Communication Preprints have been common in fields like math and physics for decades (see: arXiv.org...commented on by readers, typically peers in the field, and updated by the authors over time, providing...subscriptions to the specialized journals in your field? Post a preprint before you submit to a journal ...accessible, allowing problematic work to be quickly identified and rigorously discussed and addressed by the...

...virus, introduced to the field of fusion proteins more than thirty years ago (Field et al., 1988). Thus, ...experiments. Fluorescent proteins can also be identified using antibodies and immunohistochemistry techniques...–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson...

...In vitro tests for aggregation The tendency of purified fluorescent proteins to form homodimers or higher...what extent does homodimerization assayed with purified protein, reflect the likelihood that fluorescent...OSER) whorls are visible. These whorls can be quantified for individual cells, and the number of 'normal...under a limited number of conditions, often using purified proteins. Therefore, it is essential to verify...

... intense light (for a brief time), whereas in widefield imaging fluorophores are excited with relatively...Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion, the ...the most photostable fluorescent protein can be identified by a head-to-head comparison of several fluorescent...comparison between cyan fluorescent proteins using widefield imaging. Our choice for a photostable protein ...

...these limitations and lead to new milestones in the field. Research strategy The Research Strategy highlights...research challenge. Demonstrating expertise in the field and past successes boost chances in receiving funding... address a variety of challenges and impact the field - its encouraged to dream big! It is crucial that...into the mission of the agency and benefits the field reminds the reviewer why your project is the project...

...individual bases can be modified. Cytidine deaminase PmCDA1 Akihiko Kondo’s lab identified a cytidine deaminase...Through recombineering, the locus of interest is modified to match the repair template, and the locus cannot...more efficient, well tolerated, Cas enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...