Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 671 results for: FIE

Showing: 141 - 160 of 671 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ... region. The variable region acts as a unique identifier for individual cells. Each cell is labeled with.... The fixed region acts as a library-specific identifier and allows cells to be tagged with different ...during reprogramming. These library-specific identifiers act like a timestamp and help demultiplex the...this lineage tracing experiment, the Morris lab identified a novel gene, Mettl7a1, whose expression helped...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...small molecules since the very beginnings of the field. Those engineered sensors were designed to replace...1980s. “Synthetic dyes were the standard in the field, but there is one problem: how to get that into ... sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance energy transfer...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...selectable marker, and target gene, for example, are amplified with overlapping regions of homology and inserted...for transformation into yeast that same day.  The field of synthetic biology has led to advances in biofuel... in these methodologies offer streamlined and simplified alternatives to traditional cloning. NEBuilder...and opens the door for new opportunities in the field.  Gibson Assembly® is a registered trademark of ...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-hSyn-mCherry 50459...for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See our full biosensor... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825...

  5. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...conformational changes. These tiny movements are then amplified tens or hundreds of thousands of times by propagating...membranes outside of their natural context or 3) be modified to extend at different pH levels. Domesticating...soluble when extended (see figure to the right), we identified R-body mutants that extend at both lower and ...response to pH changes. Beyond these applications, purified R bodies could also perform as actuators in microelectromechanical...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins in the cephalochordate...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176-81. Zakeri...optimization through engineering, Ed Boyden’s lab has identified a variety of new channelrhodopsins for use as...for these effects, drawing on the rapidly growing field of optogenetics. Using a rational protein engineering...on the Golden Gate cloning method, but has been modified specifically to improve plant transgenesis. TheGreenGate...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...protocol to perform digital droplet PCR (ddPCR) for purified AAV titration....how to use droplet digital PCR (ddPCR) to titer purified recombinant Adeno-associated viral vectors (AAV...the ITR elements in the viral vectors but can be modified for other targets. The dilution series outlined...ddPCR for AAV quantification. This protocol was modified from the publication Lock et al. (2014) (Link ...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...focused on current questions and experiments in the field of synthetic biology. Founded as a nonprofit in...biology has grown into a recognized name in the field of STEM education worldwide. The BioBuilder curriculum... as a program focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific...module allows students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses...

  9. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...the exciting new field we call “precision functional genomics.” The goal of this field is to functionally...correlations between variants in a gene of poorly classified function and a well established cellular signaling...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

  10. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... use of microscopy in open science. But the new field of open-source microscopy seeks to change this. ...commercially available consumer-grade components. In this field, often referred to as 'frugal science', existing...Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy, where image processing algorithms...Nevertheless, there are still many open questions to be clarified, especially with regard to licensing models. Direct...

  11. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientific communication Preprints have been common in fields like math and physics for decades (see: arXiv.org...commented on by readers, typically peers in the field, and updated by the authors over time, providing...subscriptions to the specialized journals in your field? Post a preprint before you submit to a journal ...accessible, allowing problematic work to be quickly identified and rigorously discussed and addressed by the...

  12. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...virus, introduced to the field of fusion proteins more than thirty years ago (Field et al., 1988). Thus, ...experiments. Fluorescent proteins can also be identified using antibodies and immunohistochemistry techniques...–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson...

  13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...In vitro tests for aggregation The tendency of purified fluorescent proteins to form homodimers or higher...what extent does homodimerization assayed with purified protein, reflect the likelihood that fluorescent...OSER) whorls are visible. These whorls can be quantified for individual cells, and the number of 'normal...under a limited number of conditions, often using purified proteins. Therefore, it is essential to verify...

  14. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... intense light (for a brief time), whereas in widefield imaging fluorophores are excited with relatively...Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion, the ...the most photostable fluorescent protein can be identified by a head-to-head comparison of several fluorescent...comparison between cyan fluorescent proteins using widefield imaging. Our choice for a photostable protein ...

  15. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...these limitations and lead to new milestones in the field. Research strategy The Research Strategy highlights...research challenge. Demonstrating expertise in the field and past successes boost chances in receiving funding... address a variety of challenges and impact the field - its encouraged to dream big! It is crucial that...into the mission of the agency and benefits the field reminds the reviewer why your project is the project...

  16. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...individual bases can be modified. Cytidine deaminase PmCDA1 Akihiko Kondo’s lab identified a cytidine deaminase...Through recombineering, the locus of interest is modified to match the repair template, and the locus cannot...more efficient, well tolerated, Cas enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    ...those who are not experts in particular scientific fields. As scientists, many of us are used to being very...focused on narrowly-defined research questions in our field of interest. We often communicate in very technical...presentations. But for many people who don’t work in these fields of research, what often concerns them the most ...interest and awareness of issues outside of your field of research will allow you to better engage these...

  18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... Gaudelli et al., 2020. Directed evolution identifies adenine base editors with improved editing in...described TadA mutations (Gaudelli et al., 2017), they modified ABE7.10 accordingly which resulted in variants...the “x” is the placeholder for the number that identifies the mutation in the evolved TadA domain. Because...cells using 8 selected ABE8s and ABE7.10. They quantified genomic editing by measuring the reduction in...

  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...2019.       Direct ELISA In a direct ELISA, purified proteins or the experimental sample are bound ...instead of an antibody. Coating the wells with a purified protein allows you to characterize properties ...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received...developed two new enabling technologies for the field of synthetic biology. The first was a new class ...advancing the paper-based platform toward a more field-ready format when the Zika virus outbreak was reported...felt that, given the critical need for low-cost, field-based diagnostics, our approach could make an impact...
Showing: 141 - 160 of 671 results