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We narrowed to 475 results for: FLU

Showing: 141 - 160 of 475 results

  1. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These resources ...Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins... tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer...and mWasabi and find plasmids containing these fluorescent proteins at Addgene. 7. Designing Your Chalk...chalk talk, and tips for practicing.  6. The Fluorescent Vegetables in Aptamer Soup by Aliyah Weinstein...aptamers, which are nucleic acid sequences that fluoresce upon binding a small molecule, are often named...making scientific graphics. PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...when paired with appropriate fluorescent optics. Existing red fluorescent voltage indicators, however,...31937940. Detection of neural activity using a red fluorescent genetically encoded voltage indicator Article...voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet) with either a seven or eight...manipulations when combined with existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins...zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination. Each...variety of colors depending on the combination of fluorescent proteins expressed. CreLite-mediated recombination...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts ...constructs similarly to GFP, but commonly used red fluorescent proteins dsRed, mCherry, and TdTomato do not ...GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T-DDOGs. Like T-DDOGs...utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs and Cre-DOG are available... C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene ... Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein knockout mediated by anti-GFP ...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...chloride ion pumps, activation of which results in efflux of chloride ions, subsequent hyperpolarization,...unlike HR, this is achieved through hydrogen ion efflux; evidence suggests that Arch may more effectively...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ... vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ...amount of protein you are expecting.  Fluorescent proteins Fluorescent proteins, while more expensive, allow...typically rely on primary antibodies conjugated to fluorescent proteins, instead of the more common chemiluminescent...you to optimize for smaller proteins or lower fluorescent backgrounds. However, PVDF does add a few (short... of reporters: chemiluminescence enzymes and fluorescent proteins. Chemiluminescence enzymes are cheap...important if you want to quantify your blots). Fluorescent proteins are stable, so blots can be stored for...magnitude, which includes a wide dynamic range for fluorescent proteins. They do tend to have increased background...usually more expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes ...several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...marker. Lee et al.(1) assessed many of these fluorescent tags in S. cerevisiae, looking at their performance...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  7. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time... reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited for imaging ...context/spatial localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects...tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org...

  8. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on...transfer is between a luminescent donor and a fluorescent acceptor. TRUPATH uses an updated version of ...on Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234...n K. S. (2013) Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. ...

  10. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification. Antibodies...signal amplification occurs when HRP converts a fluorescently labeled tyramide molecule into a highly reactive...linked to residues within close proximity thus fluorescently labeling them. HRP RANbody staining results ... ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to lower background...and tissue sections Amplifying weak signals of fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution... from Penn State Need help picking the right fluorescence microscopy technique to use? Check out this ...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the...32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput ...High-throughput screening methods, such as fluorescence-activated cell sorting (FACS) helps researchers sift through...million cell types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP ...Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand break repair assay...the introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim...

  13. Control AAV Preps

    Type
    Collection
    ...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105534 ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more. Viral...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

  14. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses...these proteins are bright! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders...St-Pierre recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et ...using mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider...et al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications...

  15. Brain Armamentarium

    Type
    Collection
    ... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...quenched fluorescent ssRNA reporter is added to the reaction. Cleavage of the quenchable fluorescent RNA by...Cas13 and Cas12a, are combined with different fluorescent reporters for each enzyme. In addition, Csm6,...Csm6 and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (FQ) reporter, which will produce a...rate of ~1,250 cuts per second. A quantifiable fluorescent signal designates the presence of your DNA of...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged ... imaging in C. elegans By Alyssa Neuhaus Red fluorescent proteins like mScarlet are essential tools for...2024 under CC-BY license.  These optimized fluorescent proteins will be a useful addition to the toolkit...Comparative analysis of new mScarlet-based red fluorescent tags in Caenorhabditis elegans. Genetics, 228...wavelength (460 nm) is shorter than many popular fluorescence markers like GFP (510 nm), allowing co-imaging...

  18. Editor's Choice, July 2016

    Type
    Blog Post
    ...biological phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful...CRISPR variant going forward.   Special Delivery Fluorophore Targeting for FRET Studies While I love all of...This post describes how to target and use FRET flurophores for a variety of applications. While editing ...Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered Innovations...

  19. Microbiology Resources

    Type
    Collection
    ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

  20. Plant Plasmids and Resources

    Type
    Collection
    ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... You may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR...Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...you are looking for, such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter...
Showing: 141 - 160 of 475 results