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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 407 results for: HRE

Showing: 141 - 160 of 407 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers receive overtime payment...either all affected scientists are paid above this threshold or for tracking the hours that these scientists...

  5. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...to ensure off-target events are below a given threshold for certain experiments where they can’t be tolerated...or maybe you just can’t get them below a given threshold even when you’ve tried everything listed here....

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...ready to start selecting, testing, and optimizing three key variables of an AAV vector: serotype, promoter..., & Burke, R. E. (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    .../F0, Time-to-peak, Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025... highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1, GPC3, ...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...Academic Mentors.  What is workplace bullying? Threats to professional status – including belittling opinions...intimidating use of discipline or competence procedures Threats to personal standing – including undermining personal...

  9. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... diseases. Others were inspired by the imminent threat of climate change to study plant and marine biology...Chrousos GP, Mentis A-FA (2020) Imposter syndrome threatens diversity. Science 367:749.2-750 . https://doi.org...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... for more variables and groups. ANOVAs can test three or more groups (two samples and a control, for example...Bonferroni does this by establishing a significance threshold at a p value less than or equal to the alpha value...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ... real estate and using AAVs have in common? The three most important items when looking for a house and...Applying this mantra to AAV selection, there are three important factors related to location. Location...

  17. Typing CRISPR Systems

    Type
    Blog Post
    ... types — weird and poorly understood. There are three subtypes, A–C, and they are grouped together because...when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter ...fragments are PCR amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors...

  19. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Mycobacterium smegmatis, termed UdgX, they found three proteins whose fusion improved the CGBE: DNA polymerase...where we’ll tackle transversion editors for the three other DNA bases, bringing the dream of any-base ...

  20. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition...DNA strand are not resolved due to disorder. All three stem loops and all 20 guide bases of the RNA are...
Showing: 141 - 160 of 407 results