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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 433 results for: Hal

Showing: 141 - 160 of 433 results

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...

  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  10. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...cloned from include click beetles (Pyrophorus plagiophthalmus), railroad worm (Phrixothrix hirtus), and a... ATP No Jamaican click beetle(Pyrophorus plagiophthalamus) 544-593 64 Luciferin ATP No Railroad worms...

  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...interested in studying genes whose knockout would be lethal to cells and thus not assayable with CRISPR. Another...could culture cells in the drug of interest at a lethal dose for non-manipulated cells and then assay cells...

  13. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...engineering designs and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant...of students and teachers. It’s possible to join a half-day workshop for teachers, to schedule a three-day...

  14. Antibodies 101: ChIP

    Type
    Blog Post
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  15. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest...
Showing: 141 - 160 of 433 results