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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 185 results for: INV-E

Showing: 141 - 160 of 185 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others)...Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease... Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM...9. PMID: 26355004 Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, ...as a powerful defensive strategy against viral invaders, and this system is proving to be just as useful...CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy the phages upon re-...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One mentor isn’t enough. Here’s how I built...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...prep media, etc. "Research assistants" are more involved in the science, experimental design, and execution...genuinely excites them, and reach out to the PI to get involved. It not only demonstrates initiative and drive...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ... PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation of dopamine neurons...and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function ... flip-excision) switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation))...recognition sites, its presence induces first opsin inversion, and then lox sites excision, therefore locking...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...-312. PubMed PMID: 27087594. 16. Tycko, Josh, Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing...Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that their samples...off-target mutations are essential data points for investigators looking to definitively link genotype and phenotype...genome-wide unbiased methods. However, most investigators use single cell clones for in vitro CRISPR experiments...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs..., M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions...Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin... silencing mechanism. It is a useful tool to investigate the roles of specific genes or to knockdown genes...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...:947–951 . https://doi.org/10.1038/nbt.2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation...homology directed repair, these methods do not involve double strand break (DSB) formation and do not ...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...- it is hard to distinguish between specific, invariable expression and non-specific, background signal...

  9. Arf GTPase Family

    Type
    Collection
    ... (Link opens in a new window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright...Santy, L. C., Schürmann, A., Wilhelmi, I., Yohe, M. E., & Kahn, R. A. (2019). ARF GTPases and their GEFs...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...referred to as a minimal triad, with each component involved in GTPase signaling activity. Figure 1: Locations...

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp....lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual colonies...

  13. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Goodson, N. B., Park, K. U., Schwanke, M., Office, E., Schneider, S. R., Abraham, J., Hensley, A., Jones...CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...aide in their studies of the regulatory networks involved in retinal development the lab utilizes a CRISPR...

  14. COVID-19 Resources

    Type
    Collection
    ...2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...infection, such as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find ...protease that primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme...superfamily, binds to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L -...infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV... BSG - Wang, K et al, 2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein...

  17. CRISPR References and Information

    Type
    Collection
    ...the Jin-Soo Kim Lab . CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation (Link opens in...repression and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm...John Doench , David Root , and Hyongbum Kim Labs. E-CRISPR (Link opens in a new window) Identifies gRNA...Currently supports over 20 model and non-model invertebrate species. Developed by the Kate O'Connor-Giles...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta 5 FLJ76359, HLA-DRB HLA-E major histocompatibility complex, class I, E DKFZp686P19218, EA1.2, EA2.1...subfamily 2, group E, member 1 TLL, TLX, XTLL NR2E3 nuclear receptor subfamily 2, group E, member 3 ESCS,...PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa EP1 PTGER2 prostaglandin E receptor 2 (subtype ... HLA-JY3, HLC-C, PSORS1 HLA-E major histocompatibility complex, class I, E DKFZp686P19218, EA1.2, EA2.1...MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL, CTSL, FLJ31037, MEP ...cell lectin-like receptor subfamily C, member 3 NKG2-E, NKG2E KLRC4 killer cell lectin-like receptor subfamily...IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin heavy variable...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial ...TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His and NusA fusions...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...BiFC vector (pEarleyGate201-YN) Zeocin® is an InvivoGen trademark. Return to top Content last reviewed...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase gene allow for increased copy number in E. coli and expression of luciferase in the cytoplasm...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha...212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha...
Showing: 141 - 160 of 185 results