We narrowed to 298 results for: IRES

1. Let There Be LITE Plasmids

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   Blog Post

   ...environments. We are able to react and learn. All of this requires genes to be regulated dynamically.” To understand...

2. Phage Directory: From Phage Therapy to a Repository of Phage Information

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   ... archive phage reagents. If a phage researcher retires, it's likely that the phages are lost if they are...

3. Easing the Protein Purification Process with pCri

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   ...stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively...

4. Science as Art

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   ...The other thing photography has shown to me - it requires an awful amount of skill,  time for which most...

5. What's New in CRISPR - September 2019

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   ...gene) on the donor vector. Therefore, targeting requires two sgRNAS: one that targets the knock-in site...

6. New Norepinephrine Indicators: nLightG and nLightR

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   Blog Post

   ..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   ...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...

8. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   ...chemogenetics on the Addgene website. Optogenetics also requires that light of the correct wavelength reach receptors...

9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   ...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...

10. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

11. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    Blog Post

    ...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...

12. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

    ...about kit cost and safety.  Being a manager also requires Caitlin to look at the big picture: “It’s been...

13. Build Your CRISPR Vocabulary

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    Blog Post

    ... of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between...

14. Management for Scientists: What Makes a Good Manager Anyway?

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    Blog Post

    ...time spent at the desk, but it is worth it. This requires a focus on deliverables. It does not mean a manager...

15. Advice for Choosing a Research Project

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    ...approach. At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

16. RUBY-Red Siliques

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    ...plants, but screening these can be challenging and requires specialized equipment. Reporters for studying ...

17. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    ...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

18. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post

    ...on our blog. Image: The final and required step requires submission of an experimental image result that...

19. Plasmids 101: Origin of Replication

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    ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...

20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post

    ...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...