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We narrowed to 526 results for: MEA;

Showing: 141 - 160 of 526 results

  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...eventually called aptamers, from the Latin aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers...Making aptamers glow The flexibility of aptamers means they can be used with or as fluorophores in several...Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability and decrease background...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely...lit (risky) and a dark (protected) area. When measuring the time spent in a particular part of the apparatus...46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher. "...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression... revolutionized Neuroscience. They are the only means to target and manipulate specific cell types (populations... FLPe recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...vector and internal control A transfection control measures transfection efficiency and enables observation...transfection control is an internal control vector, which measures transfection efficiency. An internal control may...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...High-throughput DNA sequencing is then used to measure the frequency of each variant during the selection...contains their respective recognition site. This means that if the BbvCI enzyme used for ssDNA preparation...Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale...

  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... needs to be asked going forward is: How do we measure inclusion and equity? This is a difficult question...discluded/not treated equitably even when hired; well-meaning mentors often fail to judge the work of underrepresented...and participants of the symposium began providing means to answer some of these questions and provided ways...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...that you’re looking at institutes that have the means to support your work. For example, if your work ...Sometimes, “no we don’t have the funds,” really means they don’t have the money. If the department has...help you achieve your research goals, even if that means saying no to a negotiation or, in this case, to ...

  8. CRISPR Guide

    Type
    Guide
    ...based on the gRNA sequence without cleavage. This means proteins with other functions can be fused to a ...Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they will be referred...principle, the ability to induce base transversions means that any base-to-base conversion is possible with...CASTs can result in bidirectional insertions — meaning they may not be suitable for applications where...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...consequences in an experiment. For CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target...experimental results and 2) perform targeted off-target measurements at those sites to get an idea of how high the...factor, etc.) this control is usually not very meaningful. Unfortunately, there really aren’t any effective...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...abilities, or other phenotypes that can be efficiently measured at the single cell level), are separated by, for...mouse libraries available through Addgene. This means you could perform a screen with one library from...can be used: Design a screen with an easily measureable cellular phenotype (e.g. survival of a cancer...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...GPCRs in the human genome are orphan receptors meaning their ligands are unknown. Identifying new ligands...Protein A tag. The luciferase activities can then be measured and normalized to assess interactions. Higher ...of CRISPR and AID technologies provides a clever means of investigating hard-to-study genes in hard-to-... work, they used the CRISPR/Cas9 technology to measure the contribution of each gene from the model apicomplexan...

  14. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...this new green glowing protein could be used to measure gene transcription, as well as to track protein...

  15. Science Communication for Everyone

    Type
    Blog Post
    ...manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked with”...

  16. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...in neurons. Indeed, this AAV provides you with a means to specifically activate expression of any Cre-dependent...Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals...

  17. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...on the team talks and listens in roughly equal measure, keeping contribution short and sweet. One key...team. We are not talking about gossip here – this means encouraging a culture of maximum information flow...

  18. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...Addgene nearly 10 years ago, lunch felt like a family meal. My co-founders actually were my family (Kenneth... forward to gathering around a large table for a meal with my family. I am thankful for many things, and...
Showing: 141 - 160 of 526 results