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We narrowed to 848 results for: Met

Showing: 141 - 160 of 848 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... editing is a “search and replace” gene editing method in which a reverse transcriptase (RT) is fused ...

  2. Worm Expression Resources

    Type
    Collection
    ...elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic model organism...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...on factors such as selectable marker or cloning method. When using CRISPR, you will need to express both...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...standards to calibrate the numerous and disparate methods used to measure FRET. Plasmid FRET Pair C5V Cerulean...

  5. Viral Vectors

    Type
    Collection
    ...modified and amplified (through standard cloning methods). There are different types of viruses that are...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...on factors such as selectable marker or cloning method. When using CRISPR, you will need to express both...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...depend on several factors including: the delivery method, mouse strain, target cell type or tissue of interest...

  11. Molecular Biology Reference

    Type
    Guide
    ...UUG, CUU, CUC, CUA, CUG Lysine Lys K AAA, AAG Methionine Met M AUG Phenylalanine Phe F UUU, UUC Proline ...To learn more about different types of cloning methods check out our guide on molecular cloning techniques...developed the process termed Sanger sequencing, sometimes referred to as chain-termination sequencing or... making it an efficient and reliable sequencing method. Next Generation Sequencing Although Sanger sequencing... 5 factors to help you choose the right cloning method Plasmids 101: Restriction Cloning Plasmids 101:...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...fused a TALEN to the Tet1 demethylase catalytic domain, could mediate demethylation and induce transcription... fused a TAL effector to LSD1 histone demethylase to demethylate enhancer regions (Mendenhall et al., ...Tet1 demethylase Ronggui Hu’s lab has created pdCas9-Tet1-CD for targeted cytosine demethylation in mammalian... dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted demethylation is associated... dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted methylation is associated... Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24 hours rather...CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and...

  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...in their DNA methylation patterns, either large increases or decreases. Hypermethylation of CpG islands...hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification...linker is a methyllysine binding domain (chromodomain), which binds selectively to lysine-methylated peptides...the results. When methylation occurs at the histone-derived peptide, the methyllysine binding domain causes... (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27 histone methylation reporter) positions ...to recognise lysine 27 methylation. When tested in living cells where methylation of these sites was controlled...significantly different FRET levels between unmethylated and methylated states. Thus, this system is a reliable...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...efficiency and cross-talk. Methods to Measure FRET for Cell Biology Studies Several methods have been used over...many measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment... this method useful for dynamic experiments in which FRET changes are large. Two other methods are commonly... photobleaching method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching...photobleaching method The acceptor photobleaching method is simple but limited to a single measurement. This...imaging microscopy method FLIM has been developed more recently and is the most rigorous method for measuring...pitfalls and to use it at its best. What are the Parameters that Affect FRET? FRET occurs when the two fluorophores...

  15. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...and chemiluminescent methods that require specialized equipment. Chromogenic methods are not as sensitive... Because the method uses a single antibody, it is less specific than other ELISA methods. Users may also...drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the assay requires...advantages and disadvantages of each ELISA method Method Advantages Disadvantages Direct Fast and...enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen in...of each, and some considerations when using the method. ELISA basics All ELISAs follow the same overall... discuss the more common enzyme-based reporter methods. For enzymatic signal detection, users provide ...

  16. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...expression. CRISPRa methods vary in their transcriptional activators: some methods rely on fusion proteins...Newer methods for CRISPR activation While SAM, SunTag, and VPR have become the most popular methods for ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, ... in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert... 2013). In the years that followed, innovative methods greatly improved CRISPRa, expanding its practicality...al., 2015, Chavez et al., 2015). Most popular methods for CRISPR activation CRISPR activation uses dCas9...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... that locus to be methylated. This powerful tool has been used to direct methylation to and lower expression...popular biotinylation method for identifying protein-protein associations. This method uses a bait protein...enriched via biotin pull-down methods and identified using mass spectrometry. The Roux Lab’s new and improved...histidine (HIS3), leucine (LEU2), uracil (URA3), methionine (MET17), and lysine (LYS2) deficiencies, and combinations...Molecular Pathology have developed an alternative method, called STARR-seq (self-transcribing active regulatory...cell lines or transgenic animals.   Wall, et al. Methods Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila...platform). GMAP is based on the Gibson Assembly method, which allows for easy assembly of DNA fragments...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ... analysis method. ΔΔCT/CQ method One of the most commonly used methods is the ΔΔCT/CQ method, which gives... as the other most commonly used method is the Standard Curve method. See Table 2, (adapted from Wong,... for a comparison of different analysis methods.   Method Standard Curve?  Amplification Efficacy...(eds.), Quantitative Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI...(Taylor, 2017). With the right conditions and parameters, some people find it can detect even smaller ...copy of a transcript. But this sensitivity can sometimes cause as many issues as it solves, so understanding...amplicon sequence and thermodynamic and experimental parameters (neighbor stacking energies, loop entropy effects...

  19. MXS Chaining

    Type
    Blog Post
    ...imaging or flow cytometry applications in mammalian cells. The modules used in this method include fluorescent...various cloning methods are listed in Table 2. For example,  chaining-based cloning methods require that ...addgene.org. Table 3: Chaining Based Cloning Methods Method Restriction Enzymes Chaining Direction ...which is the basis for the repeatability of the method. Note that the restriction sites of the MCS are...these modular types of constructs, and different methods are suited for particular downstream applications...restriction enzyme recognition sites. Thus, chaining methods are not necessarily suitable for combining endogenous...Table 2: High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type...

  20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...original CAPTURE method. Both CAPTURE methods outperformed other 3D chromatin methods such as ChIA-PET...capture (3C) based methods, such as 5C or Hi-C (Han et al., 2018). Think of 3C methods as taking a snapshot... in situ of Regulatory Elements) method. The original CAPTURE method was published in 2017 and addressed...Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation of a stable...by BirA. From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin...factors, can increase a gene’s transcription. Sometimes enhancers are located thousands of base pairs ...data. While “pictures” generated with current 3C methods provide useful information about chromatin interactions...
Showing: 141 - 160 of 848 results