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Addgene

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We narrowed to 416 results for: Numb

Showing: 141 - 160 of 416 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    ...is particularly challenging as they are great in number, generally very small, and can contain different... showed reduced proliferation and had a greater number of red cells as measured by flow cytometry. Cells...

  3. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...is contaminated? No - what really matters is the number of hits to the same sequence (gene). In a clean... this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our threshold...

  5. Common Injection Routes in Mice

    Type
    Blog Post
    ...Deciding which type of injection to use depends on a number of factors. You have to weigh the pros and cons...what’s being injected, the type of experiment, the number of mice, the potential pain or discomfort of the...

  7. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...applications require teaching experience or high numbers of publications, which may take a longer time to...billion dollars and at least a decade of work. The number of steps, decisions and people involved is staggering...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient gene transfer....qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present...

  10. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...including gene expression profiling, studying copy number variation, and molecular diagnostics.  Digital ...concentration Gene expression profiling Copy number variations Quantify RNA levels Molecular Diagnostics...

  11. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...that you’ve printed your poster and practiced a number of times, it’s time to expertly show off your science...thirty-minute-long presentations, which constrains the number of posters you can see. If you are pressed for ...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...disciplines and scientists employ GFP for a wide number of functions, including: tagging genes for elucidating...general epitope tag for protein purification and a number of commercial antibodies to GFP are available. ...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ...inflorescences begin to grow. This will also increase the number of inflorescences overall, which may improve transformation...transgene. You can optionally remove them to reduce the number of non-transformed seeds from the pool you will...

  16. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous laureate...undergraduate education in the sciences by making a greater number of students active participants in the research...

  17. DNA Purification Without a Kit

    Type
    Blog Post
    ...Kim and Morrison method, you can just adjust the number of glass filters you use depending on the size ... DNA purification kit, you can still reduce the number of spin columns you need to complete your research...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Rev and one encoding Gag and Pol, increasing the number of recombination events necessary to create a replication-competent...lead to lower viral titers due to higher plasmid number. For more information on the key differences between...

  19. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...-10 colonies in total to test, depending on the number of background colonies on your no ligation control... insert DNA. Colony PCR will greatly reduce the number of clones you’ll need to send for sequencing, but...
Showing: 141 - 160 of 416 results