Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 538 results for: PAN

Showing: 141 - 160 of 538 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months...Jennifer. I had finally found my footing with the company. I’d identified the ways to get the most out the...One: The Ironing Board Control Center With the pandemic in full swing and everyone working from home, ...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...need them. The lab re-opened very early in the pandemic with some incredibly strict safety protocols to...

  2. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...would be from doing a remote internship during a pandemic. Before I knew how bad COVID was getting, I was...interviewed fellow Addgenies about their role in the company. One of the things I wanted to do was create content...were a part of the different departments of the company. I asked questions relating to their roles at Addgene...month, I can say with certainty that Addgene is a company that cares - not only in terms of its employees... Addgenies. I could take an active role in the company and not just feel like an additional person each...

  3. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital...from.” Working at eScience Labs, an educational company that creates hands-on science lab kits and digital...called “Teaching Your Research.” This course has participants take their research from the bench to the classroom... editing.” It was for Cactus Communications, a company that provides scientific editing services. “I was...what it’s like to work for a science education company. And make sure to subscribe to the Career Channel...

  4. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...Table 1: Taking it a Step Further - Big Company v. Small Company Big (e.g. Pharma) Small (e.g. Biotech...advancement in one company, good performers in for the long haul Limited movement in one company, likely will...More sources of training and resources within the company Must find own sources of knowledge often externally...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...ready-to-use viral preps and we’ll be continually expanding our collection. We’ll provide a brief rundown ...find our updated viral vector pages as well as expanded virus protocols, information on how we produce...email to [email protected]. Your questions help us expand and improve our resources for future users. We’...

  6. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...service supporters We would like to thank the companies who supported this project with funding. Alnylam...hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly...distribution, this project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    ...want my company to pay for me to attend a conference. How can I negotiate to show the company that investing... How can I negotiate my starting date with the company so they know I’m excited while still being able...want to learn more about a different area of the company. How can I negotiate with my manager to gain experience...but I need help from another person/team at the company. How do I negotiate with the person/team for help...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA...With the expanded targeting ability using both systems, the authors look forward to the expanded use of ...precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can ...

  10. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...necessarily mean that you are a future employee of the company. It does, however, offer you an opportunity to ...like to work, get online and take a look at the company or institution’s career page, where you’re likely...openings. A good approach is to do this first for companies at which you’ve already made a personal connection...result in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying...development center, if one exists. Get online! Review company and institution career pages. Make yourself visible...

  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...unique solutions to the same problem. If your lab/company/team is still working towards a culture of encouraging...just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone could fill out the...of which, when implemented, impacted the entire company. It is important to note that the person who submitted... is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism When ...

  12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ....1007/978-1-0716-4358-7_20  Karmakar, S., Panda, D., Panda, S., Dash, M., Saha, R., Das, P., Avinash,...different TAM (5′-TTAT-3′). The unique TAM requirements expand the number of genomic sites that can be targeted...continues to evolve, we anticipate its use will expand into diverse plant and animal systems, potentially...References and Resources References Karmakar, S., Panda, D., Baig, M. J., & Molla, K. (2025). A unified ...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice of homology...need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller fragments...Y, Lai Y, Duan D (2008) A Hybrid Vector System Expands Adeno-associated Viral Vector Packaging Capacity...19:36–45 . https://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone...194–211 . https://doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ...in humans have a functional homolog in the fly (Pandey and Nichols, 2011). A huge advantage of using the...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using plasmids....β-cells that originate in different regions of the pancreas and produce different levels of insulin (Hesselson...Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling...113.8 (2016): E1074-E1081. PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease...

  15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...-actin in panel A. Indeed, the quantification in panel B is not especially linear. In panel C, two different...with permission from Elsevier. In this figure, panels A–B show the housekeeping protein approach. By ...measurements are linear. Note the different y-axes between panels B and C, indicating that β-actin is less sensitive...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...liver, muscle, lung, CNS, kidney, retinal, and pancreatic cells (Ahuja et al., 2025; Issa et al., 2023;...been shown to transduce skeletal muscle, cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells...been shown to transduce liver, skeletal muscle, pancreatic, and retinal cells (Issa et al., 2023). AAV11... Kidney AAV8 Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8...2025). Evaluation of transduction efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes...

  17. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...

  18. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship... assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...

  19. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however...Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics to developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To...personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is it to ...

  20. Reflections and Looking Ahead

    Type
    Blog Post
    ...science more accessible and reliable. We continue to expand our educational content supporting the tools in...the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly characterized...Packaged on Request, a service that dramatically expands and simplifies your viral vector options. Almost...Through our growing community on Bluesky, our expanding educational resources, and our commitment to global...
Showing: 141 - 160 of 538 results