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Showing: 141 - 160 of 535 results
  1. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...ready-to-use viral preps and we’ll be continually expanding our collection. We’ll provide a brief rundown ...find our updated viral vector pages as well as expanded virus protocols, information on how we produce...email to [email protected]. Your questions help us expand and improve our resources for future users. We’...
  2. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...
  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...service supporters We would like to thank the companies who supported this project with funding. Alnylam...hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly...distribution, this project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression...
  4. Transferable Skills: Negotiation

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    Blog Post
    ...want my company to pay for me to attend a conference. How can I negotiate to show the company that investing... How can I negotiate my starting date with the company so they know I’m excited while still being able...want to learn more about a different area of the company. How can I negotiate with my manager to gain experience...but I need help from another person/team at the company. How do I negotiate with the person/team for help...
  5. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA...With the expanded targeting ability using both systems, the authors look forward to the expanded use of ...precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can ...
  6. Transferable Skills Guide: Creativity

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    Blog Post
    ... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...unique solutions to the same problem. If your lab/company/team is still working towards a culture of encouraging...just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone could fill out the...of which, when implemented, impacted the entire company. It is important to note that the person who submitted... is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism When ...
  7. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ....1007/978-1-0716-4358-7_20  Karmakar, S., Panda, D., Panda, S., Dash, M., Saha, R., Das, P., Avinash,...different TAM (5′-TTAT-3′). The unique TAM requirements expand the number of genomic sites that can be targeted...continues to evolve, we anticipate its use will expand into diverse plant and animal systems, potentially...References and Resources References Karmakar, S., Panda, D., Baig, M. J., & Molla, K. (2025). A unified ...
  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...necessarily mean that you are a future employee of the company. It does, however, offer you an opportunity to ...like to work, get online and take a look at the company or institution’s career page, where you’re likely...openings. A good approach is to do this first for companies at which you’ve already made a personal connection...result in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying...development center, if one exists. Get online! Review company and institution career pages. Make yourself visible...
  9. Five Popular Model Organisms

    Type
    Blog Post
    ...in humans have a functional homolog in the fly (Pandey and Nichols, 2011). A huge advantage of using the...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using plasmids....β-cells that originate in different regions of the pancreas and produce different levels of insulin (Hesselson...Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling...113.8 (2016): E1074-E1081. PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease...
  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post
    ...sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice of homology...need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller fragments...Y, Lai Y, Duan D (2008) A Hybrid Vector System Expands Adeno-associated Viral Vector Packaging Capacity...19:36–45 . https://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone...194–211 . https://doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C...
  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...-actin in panel A. Indeed, the quantification in panel B is not especially linear. In panel C, two different...with permission from Elsevier. In this figure, panels A–B show the housekeeping protein approach. By ...measurements are linear. Note the different y-axes between panels B and C, indicating that β-actin is less sensitive...
  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...liver, muscle, lung, CNS, kidney, retinal, and pancreatic cells (Ahuja et al., 2025; Issa et al., 2023;...been shown to transduce skeletal muscle, cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells...been shown to transduce liver, skeletal muscle, pancreatic, and retinal cells (Issa et al., 2023). AAV11... Kidney AAV8 Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8...2025). Evaluation of transduction efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes...
  13. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post
    ...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...
  14. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

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    Blog Post
    ...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship... assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...
  15. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

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    Blog Post
    ...Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however...Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics to developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To...personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is it to ...
  16. Reflections and Looking Ahead

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    Blog Post
    ...science more accessible and reliable. We continue to expand our educational content supporting the tools in...the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly characterized...Packaged on Request, a service that dramatically expands and simplifies your viral vector options. Almost...Through our growing community on Bluesky, our expanding educational resources, and our commitment to global...
  17. Working 9-5: an Addgene Co-op

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    Blog Post
    ...training, I was gradually introduced to Addgene as a company and given more details on what my position entailed.... The official training process was lined out to span the first three months of my six month employment...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...they do and how it relates to other teams at the company. This provides new (and old) employees with information...
  18. RUBY-Red Siliques

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    Blog Post
    ...Bottom panel, right) A highly expressing RUBY plant is a vibrant red/magenta while (bottom panel, left)...plant beginning to germinate. (Top right, second panel) Transformed seedlings can be selected without antibiotics...developing seeds from a homozygous RUBY plant. (Bottom panels) RUBY expression can vary from different transformation...
  19. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...viewed publicly and stored in one place if the participants used the conference hashtag after the conference...
  20. How to Lead a Great Meeting

    Type
    Blog Post
    ...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,... if any additional resources are needed. All-Company Meetings: As your organization grows, so will the...to address one point aloud. This keeps our all-company meetings short, informative, and pleasant. Email...timely feedback on each other’s projects. For all-company meetings, monthly still works best. Figure out ...
Showing: 141 - 160 of 535 results