We narrowed to 257 results for: PORA

1. Visualizing Genomic Loci with CRISPR-Sirius

   Type

   Blog Post

   ...robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements...

2. Creating accessible biology activities in schools with BioBits

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   Blog Post

   ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...

3. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...colors of orange and white. The decor was very contemporary and futuristic with block chairs in the hallway...

4. Pushing the Limits of DNA Assembly

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   Blog Post

   ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...

5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   Blog Post

   ...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...

6. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

   ...you to systematically study gene function with temporal control and cell-type specificity! The Gal4/...

7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ... and the targeting guide RNA into cells by electroporation. Results from our lab and others (Lin et al...

8. Resume Writing for Non-academic Science Careers

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   Blog Post

   ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...

9. Advice for Scientists Starting a Lab

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   Blog Post

   ...spending more time in his office, he gradually incorporated regular group meetings, journal clubs, and focused...

10. New and Upcoming Viral Vectors - May 2020

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    Blog Post

    .... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for...

11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    ...  To create GEmCherry2, the Denham lab first incorporated an out-of-frame CRISPR genomic target region...

12. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...the research lifecycle when suggestions can be incorporated into the proposed experiment. Furthermore, the...

13. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    ...decided to create a hybrid system where we could incorporate cold-tolerance inducing genes within a regular...

14. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post

    ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

15. Viral Vectors 101: Viral Applications

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    Blog Post

    ... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

16. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

17. Clear and Accessible Writing

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    Blog Post

    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ...

18. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...light to control signaling provides greater spatiotemporal resolution than pharmacological or genetic ...

19. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

20. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...