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We narrowed to 651 results for: PPL

Showing: 141 - 160 of 651 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and Pandey et al., 2023... CreVLPs, and eVLPs for in vivo and in vitro applications.   Figure 2: Modular features of DIRECTED...approach for experimental therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D...interactions or map a local proteome, but some applications have been challenging or inaccessible. The Ting...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record...Lab’s use of Anti-PSD-95 [K28/43R] under the Application section of the Addgene antibody page, and learn...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...genomic site of interest. The donor template is supplied separately. To use this system, you’d first introduce...to generate new pEcgRNA. pEcCas/pEcgRNA can be applied not only in coli K-12 strains, but also in E. coli...genome editing in microorganisms which could be applied to optimization of genome editing tools.   Jieze...engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of...research focuses mainly on the development and application of a multiplex genome editing system based on...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...activity of CcdB. Applications of ccdB in plasmid cloning Perhaps the most widely used application of CcdB is...used as biological tools for a wide range of applications in molecular biology or in vivo studies. Let...types of plasmid addiction systems and their applications. The discovery of toxin-antitoxin systems Toxin-antitoxin...construct. Other toxin-antitoxin systems and their applications MazE/MazF Aside from CcdB/CcdA, there are many...were retained in 75% of cells. They were able to apply this system as an orally administered diagnostic...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing...delivery is not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This...is still not considered safe for therapeutic applications. piggyBac (PB) transposon: The PB transposon...express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus is a single... a safer approach for developing therapeutic applications, but it can take a long time to reprogram cells...there’s no universal method that can handle all applications of iPSCs, but there should be at least one method...Induced pluripotency: history, mechanisms, and applications." Genes & Development 24, no. 20 (2010): 2239...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...years ago, I decided to write a "how to" manual on applying for faculty positions in biomedical science. My...talents and ideas. Some key points from my ebook, "Applying for a Faculty Position: the View from Both Sides...summarized below. Check Out Erik Snapp's Full "Applying for a Faculty Position" eBook Here 1. Have a competitive...experts in your field. Not true. Even if you are applying to a department in your specialty area, it's a...reserved for questions. 5. Understand the job you are applying for It's not about you and how you will finally...interviews. For point of reference, I submitted my applications all by snail mail and only had phone interviews...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...or free amines of proteins. Depending on the application, they can be membrane-permeant or membrane-impermeant...are positive or negative for the dye. The main applications of dye stains in flow cytometry are cell proliferation...with a high resolution for specific downstream applications, while maintaining a clean cell surface, dump...and bind DNA or free amines, depending on the application. Paul Heisig is a Research Associate in the lab...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including...plague these animals even if their diet was being supplemented with biotin. Thus something in the egg white... feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application of avidin-biotin technology to improve cell adhesion...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...current CRISPR research focuses on its potential applications for human medicine (Waltz, 2016), the potential...selected the correct CRISPR components for your application, it is time to deliver these components to your...detergent, Polyethylene Glycol (PEG), although the application of this approach is limited to protoplast cells...find many plasmids for a wide variety of CRISPR applications in plants available through Addgene. In addition...N (2016) The CRISPR/Cas Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: ....10.1073/pnas.1818290116 Zhu H, Li C, Gao C (2020) Applications of CRISPR–Cas in agriculture and plant biotechnology...

  9. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...very little data on which might work in your application. It can be quite difficult to decide on which...be searched and sorted by gene/target, name, application, pass/fail rating, sample species, antibody species... how the antibody performed in the submitted application, along with a brief description of the experiment... section will vary slightly depending on the application — flow cytometry, western blot, immunohistochemistry...data showing how the antibody worked in your application. The submission process is easy, with simple,...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...of the protein of interest. An electric current applied to the gel causes the negatively charged proteins...with blotting paper and an electric current is applied causing proteins to migrate out of the gel and ...search, first pay attention to the validated applications listed on the vendor’s website and choose an...western and vice versa.  In addition to validated applications, pay close attention to the controls listed ...choose antibodies that are validated for your application and test the antibody in your specific system...to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones for lentivirus...Cre-dependent expression of a GMAP inserted gene. The applications for such a modular system are endless and, as...genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional 6 gRNAs...connectors” that link tags to the targeted gene are supplied to the reaction from a prebuilt donor plasmid ...sequences for homology arms and the guide RNA are supplied as annealed synthetic oligos - eliminating the...mutations, and is suitable for high throughput applications. Trepte P, et al. J. Mol. Biol. 2015. PubMed...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...for this co-op, I was immediately interested in applying. This had stood out to me because it gave me a...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...

  13. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...technique published every week! One of the newest applications is a colorful system that uses fluorescently...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard... et al. anticipate that the technique will be applicable to studies of cell cycle progression, epigenetics...to external stimuli. It could also have major applications to cancer, for example, the visualization of...

  14. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...different CRISPR applications in plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tag to remain on your protein for downstream applications Below, we describe a few of the most common...not to perturb protein function for downstream applications. Tag cleavage must be performed after primary...also provides a visual detection mechanism via applications such as western blot and immunofluorescence....dimerizes in solution, which can affect downstream applications. Also, purifying GST by affinity chromatography... are generally best suited for their primary application. Ready to start tagging? We are here to help ...

  16. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

  17. What the HEK?

    Type
    Blog Post
    ...isolating the cell line all the way to modern applications. What the HEK is it?   HEK cells were first...cell biology, there are thankfully many other applications that they are useful for! What the HEK is it...cell line and we will highlight some of those applications here.   Virus production HEKs are the go-to ...proteins at high levels. In a case where clinical applications of recombinant proteins are necessary, HEKs ...significantly reduces the cost of production. Research applications There are two areas of research where HEK cells...

  18. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...for, we wanted to be certain that this supplementation strategy would have no effect on the purity or infectivity... we will be spending less time and resources resupplying our current inventory and more time providing.... "Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology...

  19. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... be applied to dozens of other published FRET-based sensors. For example, his team is now applying it ...Because they are chemical compounds, they have to be applied or injected, and they don’t always end up where...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be... large dynamic range,” he says. Merkx recently applied this “trick” to the development of additional color...

  20. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...Let’s dive in and take a look at some of these applications. Western blot Western blots are used to detect... by binding to cell surface receptors. These applications are typically used for investigating signaling...are out there, and how many different research applications they can be used for. Addgene’s Antibody Plasmid...
Showing: 141 - 160 of 651 results