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We narrowed to 653 results for: PPL

Showing: 141 - 160 of 653 results

  1. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...very little data on which might work in your application. It can be quite difficult to decide on which...be searched and sorted by gene/target, name, application, pass/fail rating, sample species, antibody species... how the antibody performed in the submitted application, along with a brief description of the experiment... section will vary slightly depending on the application — flow cytometry, western blot, immunohistochemistry...data showing how the antibody worked in your application. The submission process is easy, with simple,...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...of the protein of interest. An electric current applied to the gel causes the negatively charged proteins...with blotting paper and an electric current is applied causing proteins to migrate out of the gel and ...search, first pay attention to the validated applications listed on the vendor’s website and choose an...western and vice versa.  In addition to validated applications, pay close attention to the controls listed ...choose antibodies that are validated for your application and test the antibody in your specific system...to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones for lentivirus...Cre-dependent expression of a GMAP inserted gene. The applications for such a modular system are endless and, as...genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional 6 gRNAs...connectors” that link tags to the targeted gene are supplied to the reaction from a prebuilt donor plasmid ...sequences for homology arms and the guide RNA are supplied as annealed synthetic oligos - eliminating the...mutations, and is suitable for high throughput applications. Trepte P, et al. J. Mol. Biol. 2015. PubMed...

  4. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...for this co-op, I was immediately interested in applying. This had stood out to me because it gave me a...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...

  5. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...technique published every week! One of the newest applications is a colorful system that uses fluorescently...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard... et al. anticipate that the technique will be applicable to studies of cell cycle progression, epigenetics...to external stimuli. It could also have major applications to cancer, for example, the visualization of...

  6. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...different CRISPR applications in plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tag to remain on your protein for downstream applications Below, we describe a few of the most common...not to perturb protein function for downstream applications. Tag cleavage must be performed after primary...also provides a visual detection mechanism via applications such as western blot and immunofluorescence....dimerizes in solution, which can affect downstream applications. Also, purifying GST by affinity chromatography... are generally best suited for their primary application. Ready to start tagging? We are here to help ...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

  9. What the HEK?

    Type
    Blog Post
    ...isolating the cell line all the way to modern applications. What the HEK is it?   HEK cells were first...cell biology, there are thankfully many other applications that they are useful for! What the HEK is it...cell line and we will highlight some of those applications here.   Virus production HEKs are the go-to ...proteins at high levels. In a case where clinical applications of recombinant proteins are necessary, HEKs ...significantly reduces the cost of production. Research applications There are two areas of research where HEK cells...

  10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...for, we wanted to be certain that this supplementation strategy would have no effect on the purity or infectivity... we will be spending less time and resources resupplying our current inventory and more time providing.... "Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology...

  11. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... be applied to dozens of other published FRET-based sensors. For example, his team is now applying it ...Because they are chemical compounds, they have to be applied or injected, and they don’t always end up where...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be... large dynamic range,” he says. Merkx recently applied this “trick” to the development of additional color...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...Let’s dive in and take a look at some of these applications. Western blot Western blots are used to detect... by binding to cell surface receptors. These applications are typically used for investigating signaling...are out there, and how many different research applications they can be used for. Addgene’s Antibody Plasmid...

  13. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem is…how... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced...they would use their logic and strategy skills to apply those concepts and understand how therapeutic strategies... BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology...opposing team. In the end, the students had fun while applying most of the concepts required by the course and...

  14. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...research box, send out some feelers, apply to programs, and apply for travel grants. Good luck and happy...visiting positions within defined labs which you can apply to. These structured types of placements often have...are your options if neither of these situations apply to you? Once you’ve identified one or more labs ...mentor can help get the ball rolling! If you are applying to a structured program, they likely have guidelines...

  15. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...together a grant application? Are you familiar with the publishing or patent application processes? How ...How can you stand out among all the candidates applying for the position?   Here are a few things to consider...makes sense for your skills, and the job you are applying for. Also remember that a resume is typically ...have done is closely related to the job you are applying for, in which case talk about your research, as...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference... 96-well plate options for medium-throughput applications. For high-throughput options, several manufacturers...

  17. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...and deliver any cargo you fancy (size limits do apply, however.) Why use a virus? Viruses are essentially...low immunogenicity which makes them ideal for applications with whole organisms. Finally, viral delivery...delivery is typically straightforward across applications, while electroporation of a human, for example, isn... (~4.5kb From basic science to clinical applications, viruses are true bench to bedside tools. Keep...our upcoming blogs on virus safety and viral applications to help you start (safely!) thinking about the...

  18. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...definition of “problem solving skills” is the ability to apply the scientific method to problems in a variety of...strong problem solving skills is just a matter of applying that framework to the myriad challenges we encounter...ask them to explain their logic. Or you can try applying the scientific method to everyday problems in ...to practice problem solving skills and learn to apply them flexibly in a range of contexts. As such, scientists...remember that you have these skills when you start applying and interviewing for new positions. Highlighting...

  19. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...

  20. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...review how these indicators work, their general applications, and why viruses are essential to their use ...improvement. These sensors still have many useful applications, though, and if you’re in the market for a VSD...tissues depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure...your cell type of interest. For in vivo imaging applications, the ability to direct your voltage sensor selectively...genetically encoded voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019....
Showing: 141 - 160 of 653 results