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Showing: 141 - 160 of 362 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted... into three distinct types and subtypes using polythetic classification based on evolutionary similarity...

  2. Control AAV Preps

    Type
    Collection
    ...Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Membrane-Rupturing Microscopic Tools In this post, Jessica Polka, formerly a postdoctoral researcher in the Silver... other chemical fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH...

  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and ligate mismatched...primer design based on fragment sequences and the polymerase used in the amplification. This tool allows instant...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively correlated... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

  12. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... depending on the primer sequence and the NEB polymerase used. Overall, while much of the information ...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

  16. Antibodies 101: ChIP

    Type
    Blog Post
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...shoulder for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip...organizational work. LinkedIn is not the platform for politics, family updates, or click bait of any kind. For...

  18. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...

  19. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert... in the intersection between science and public policy. Follow David on Twitter @driglar.     References...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View...
Showing: 141 - 160 of 362 results