...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 ...Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter CMV T7 polH GAL...132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 132393 AAVS1...AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 133320 pHBS834...177653 pT7II-2N4Rtau MAPT T7 Parkinson's, FTD Jeff Kuret 177840 pCDNA3-IgL-C99-T4L-rTA APP CMV Alzheimer'...

...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...expand monoclonal cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will... Kim C, Boulanger CM (2012) Flow cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology...

...Combinatorial strategies can also be used for retrograde tracing: visualizing neurons that received information...region. In the example shown in Figure 1, injected retrograde AAVs at one location result in opsin expression...viral vector related blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV ...guide Learn more about our viral service Find retrograde AAVs at Addgene ...

...the blue "Save Verified Data and Return to Article" button to return to the table listing all of the plasmids...the full plasmid sequence can be assembled or theoretical — it does not have to be entirely verified by...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...process for all of the plasmids in your deposit, return to the Add Plasmids page and click the blue "Submit...

...derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small...years after their initial publication, the Liu lab returned to the reverse transcriptase domain for optimization..., A., Gao, X. D., Raguram, A., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar...w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M., ...

...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...This technique is very similar to the widely used FRET, but it eliminates the need for an excitation light...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...Check Out All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...

... be fused to other proteins, and these fusions retained the ability to bind GFP. The idea of using GFP...T-DDOGs, GFP, and a reporter construct into the mouse retina. GFP expression robustly activated the reporter...Cre-DOG is both robust and specific. When tested in retinal electroporation studies, DsRed reporter expression...studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make ...

...Smith et al., 2024). They then used single-molecule FRET to study the intramolecular dynamics of the proteins...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein...

...cause an effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy...excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the two FPs...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins interact...

...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

...domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been... of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions between...concentrations and pH, using a range of strategies such as FRET, calmodulin, and others. Review Addgene's collection...

...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60...be easily expressed and purified from E. coli, retains important functional properties, and is amenable...In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is...

... tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays ...Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling...

...evaluated set of values.  However, to evaluate the interpretation of the experiment would require access to each...no one is suggesting all data be stored in a retrievable format, we too rarely preserve “raw” data of ...journal repository, they are likely too large to be retrieved by others. Other new data initiatives are also...addition to enhancing veracity of collections and interpretation of data, publishing complete data, rather than...

...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...(2013) Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https...truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease. Hum Mol ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...

...For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with...cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...

...or PNS transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be...typically administered in the lateral tail vein or retro-orbital sinus. IT injections are administered in... Chen et al., 2022 PAL2 CNS: cerebrum; neuroretina Macaques N/A Stanton et al., 2023 AAV-MDV1A...could be used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic...macaques. It also effectively transduces the neuroretina. PAL2 detargets the liver and has increased DRG...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper...

...pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from pXL yes, activate S. pyogenes EGFP Luikart...Luikart pRubiC-T2A-Cas9 75347 Mammalian/Retroviral from pXL yes, activate S. pyogenes mCherry Luikart pDECKO_mCherry...