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We narrowed to 983 results for: Ran

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Showing: 141 - 160 of 983 results

  1. Retrograde AAV viral preps

    Type
    Collection
    ...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  2. Microbiology Resources

    Type
    Collection
    ...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus ...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

  3. Worm Expression Resources

    Type
    Collection
    ...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other...

  4. Promega Plasmid Collection

    Type
    Collection
    ... educational resources, and efficient material transfer solutions that help advance science while reducing...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...specialized Promega technologies that support a range of applications. The technology includes NanoLuc...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous ...NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular proximity...

  5. Lentiviral Prep Service

    Type
    Collection
    ... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

  6. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...  References and Resources References Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N....the safety of their intervention, the team led by Kiran Musunuru and Rebecca Ahrens-Nicklas administered...disorder survive long enough to receive a liver transplant — creating intense time pressure for the research...al., 2017). These editors did not create double-stranded breaks in the DNA backbone, making them significantly... repaired with an A→G edit on the complementary strand. But the invention of base editing doesn’t necessarily...cause off-target effects in a variety of RNA transcripts independent of Cas9 targeting (Rees et al., 2019.... M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...cellular activity. For example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

  10. TALEN Engineering

    Type
    Collection
    ...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

  11. Institute for Protein Innovation

    Type
    Collection
    ...researchers with powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity...pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse... of glypican-3 (GPC3). The protein carries two heparan sulfate chains and is tethered to the cell surface... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

  13. mTOR Pathway

    Type
    Collection
    ...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

  14. Arf GTPase Family

    Type
    Collection
    ...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid...2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular ...., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi,...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

  16. Biosensor AAV Preps

    Type
    Collection
    ...dependent 1 Francois St-Pierre 179463 pAAV-hSyn-JEDI-2P-Kv-WPRE Syn JEDI-2P none Constitutive 9 Francois St-Pierre...pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE EF1a JEDI-2P none Cre dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a...pAAV-hSyn-JEDI-2P-WPRE Syn JEDI-2P none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  17. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

  18. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

  19. Cancer Research Plasmids and Resources

    Type
    Collection
    ...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with...

  20. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...
Showing: 141 - 160 of 983 results