... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development...examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases...vectors designed to streamline the production of transgenic zebrafish for studying neurodegenerative disorders...

...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme...

...CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator system with two components, the dCas9...sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component provides the ...dCas9 activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA ...Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST...endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H,...H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Oct;...

...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...cases Rett syndrome can be inherited. In females, random X inactivation results in approximately half of...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

...glycoprotein (G) gene, which is not required for the transcription or replication of the genome within infected...infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham et al., 2007b...essential G protein is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell... cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is released ...Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...56,762 (Set A) 56,476 (Set B) Broad GPP activation Caprano p65-HSF 92383 (Set A) 92384 (Set B) Activation ...164084 Knockout Human Sabatini 3rd 10 7,501 Human Membrane Protein Activation Library 113345 Activation Human...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...Human Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library 162275 Knockout Human...Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout...

...optimization of an orthogonal translation system with enhanced biological tolerance. Mohler K, Moen JM, Rogulina...phosphorylation is one of the most abundant forms of posttranslational modifications in cells and research into its..., Science 2013) and improving the orthogonal translation system to make singly or multiply phosphorylated... ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1* (V70) 188537 ). These reagents... with the optimized phosphoserine orthogonal translation system ( 68292 ), they were able to demonstrate...

...used to transform iPSCs and fibroblasts into neurons and more. New and Noteworthy: Study aberrant axon initial...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

...easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture...leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated fusion of...leader sequence (L) for translocation of the light chain across the ER membrane. Downstream of the insert... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G...high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived V L and V H domain PCR products...

...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...

...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus ...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other...

... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...