..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...(Quadros et al., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses ...

...R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine...

...Dr. Theodor Escherich, the scientist who first described them in 1885. E. coli are mainly found in the ...Expression Systems. Check out our companion post describing protein expression strains, where we go over ...

...are based on the pTT3 plasmid backbone which is described by Durocher et al. Nucleic Acids Research 2002...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...

...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse ...'d use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene plasmids...

...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric...

...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

...for and how can you do it well? Below, I briefly describe some tips for a successful postdoc fellowship.... there a tractable question or are you simply describing a phenomenon? Exploration can be important, but...

...targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform...

...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical...

...expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene/Insert PI ...

...see: Uhlen et al., 2016). In this post, we will describe the approaches that can be used to determine selectivity...recognized by either antibody was not shared. Tables describe that the antibodies both recognized the target...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...

... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

...citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...plasmid tools that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab...

...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...

...Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the lab to ask...

...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...